COMPARATIVE ANALYSIS OF PHYTOCONSTITUTIONAL AND PHARMACOLOGICAL ACTIVITIES OF DIFFERENT SEASONAL TEA LEAVES EXTRACTS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2015, Vol 6, Issue 7
Abstract
Methanolic extract of tea leaves (Camellia sinensis Linn.) of autumn and rain collections (SA and SR respectively) were investigated for in vitro antioxidants, antibacterial and wound healing activities to correlate with the impact of seasonal variation on phytoconstitutional content and commercial disparity. Both the extracts were subjected for quantitative and qualitative analysis and different concentrations (SA100, SA200, SR100 and SR200) were investigated for in vitro antioxidant activity performing DPPH, super oxide anion, hydrogen peroxide, hydroxyl radical scavenging assay and measurement of reductive ability. The antibacterial activity of SA and SR was evaluated against eight human pathogens (two Gram positive and six Gram negative) using disc diffusion method and minimum inhibitory concentration of each was determined. The influence of SA and SR in 250 and 500 mg/Kg body wt. on rate of wound closure were investigated using the excision wound model on female wister rats (Sprague Dawley) and histological investigations of treated and untreated wound tissues were performed to observe epithelialisation, fibroblast proliferation, neovascularisation, neutrophil infiltration, and collagen deposition. The SR extract showed higher antioxidant activities compared to SA. Both the extracts showed significant antibacterial activities. The topical administration of SR500 caused significantly faster healing (99.05%), more epithelialisation, less neutrophils infiltration, higher deposition of collagen and better angiogenesis in wound area as compared to the SA500 and standard. These results limpidly showed that the SR extract possess superior wound healing properties due to its higher anti oxidant and better microbial spectrum coverage probably by containing sesquiterpene derivative and higher flavonoid analogues.
Authors and Affiliations
Sayantan Maitra, Mahananda Sarkar, Prova Biswas, Arnab De, Sudipta Kaity, Amalesh Samanta
Keywords
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- EP ID EP106417
- DOI 10.7897/2230-8407.06791
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