Comparative Efficacy of Freshly Prepared and Stored Disinfectants in Working Dilutions by in Use Test
Journal Title: Scholars Academic Journal of Biosciences - Year 2017, Vol 5, Issue 9
Abstract
Disinfectants are used extensively in the homes, healthcare settings, laboratories and industries for a variety of topical and hard surface application particularly as an essential component of infection control practice with a purpose of preventing infections. Disinfectants used in hospitals and laboratories must be tested periodically to ascertain its potency and efficacy. The study was aimed to evaluate the efficacy of some locally available disinfectants and also study the stability and long term effectiveness of such disinfectants at their working concentrations. A total of six disinfectants/ antiseptics specimens were collected from various areas in hospitals like clinical departments including Out Patient Department (OPD), Indoor Patient Department (IPD) and Intensive care unit of YMCH. A 10µl of the 0.5 McFarland bacterial culture (ATCC strains of Escherichia coli, Staphylococcus aureusand Pseudomonas aeruginosa) was suspended into the 5ml of disinfectant solution at working dilution, after immediate preparation and after 7 days and inoculated on nutrient agar. Differences in the number of colony forming units were noted on both plates with and without disinfectant to know microbicidal efficiency (ME) of the disinfectant. Out of all disinfectants/ Antiseptics tested Glutarex, Rapid multi-enzyme cleaner, Avagard and Dettol were most effective against all organisms and yielded no growth on both nutrient agar plates.On the other hand Savlon and fresh day disinfectants were not effective even in freshly prepared working concentrations, and had decreased efficacy after 7 days of storage.The test disinfectants used in this study have been confirmed to be very effective as it has demonstrated that they have both antibacterial and antifungal efficacy. Recommended concentrations based on Suspension test apply only to freshly prepared solutions but the solutions are likely to be kept for more than 24 hours in a health care setting hence it is advisable to test the efficacy of working dilutions of disinfectants after storage of more than 24 hrs.
Authors and Affiliations
Dr. Sateesh. K, Dr. Sathya Anandam, Dr. Vidya Pai
Keywords
Related Articles
- EP ID EP398386
- DOI -
- Views 126
- Downloads 0
Evaluation of Effect of Education on parental anxiety before and after of voiding cystourethrogram
Voiding cystourethrogram is the golden test for diagnosis of bladder reflax to ureter. Increase of anxiety has interference with ability of parents for helping to children. Our study was done with goal of taking an appro...
Triphytochemistry and in vitro antibacterial activity of root extracts Cochlospermum planchonii Hook f. ex. Planch (Cochlospermaceae) on multireristant strains.
Cochlospermum planchonii is a food, medicinal and dye plant known in Ivory Coast under the common name "Kpôlorgô" in Sénoufo. It is widely used in traditional medicine in areas of sub-Saharan savannahs Ivorian and West A...
The first report of the life cycle of Sarcophaga (L) dux on dead reptilian carcass: Their application as forensic indicators
Insects identified on corpses have tremendous application in forensic sciences. In this article, we report, for the first time, in India, the complete life cycle of Sarcophaga (Liosarcophaga) dux Thomson (1869), identif...
Toxicity of Neem Based Pesticides (Nimbecidine and Neemazal) On the Cotton Pest, Earias vittella
In the present study, the toxic effect of Nimbecidine and Neemazal on the cotton pest, Earias vittella was evaluated. For Neemazal T/S the doses used were 1.0, 1.5, 2.5 and 5.0 g/insect whereas for Nimbecidine 0.9, 1.1,...
Bacteriological analysis of plastic and wood chopping boards
Abstract: This study was conducted to compare the microbial load of used plastic and wood chopping board samples and to determine the presence of Salmonella sp. and other Enterobacteriaceae species from the samples. Plat...