Triphytochemistry and in vitro antibacterial activity of root extracts Cochlospermum planchonii Hook f. ex. Planch (Cochlospermaceae) on multireristant strains.
Journal Title: Scholars Academic Journal of Biosciences - Year 2014, Vol 2, Issue 10
Abstract
Cochlospermum planchonii is a food, medicinal and dye plant known in Ivory Coast under the common name "Kpôlorgô" in Sénoufo. It is widely used in traditional medicine in areas of sub-Saharan savannahs Ivorian and West African. The aim of our study was to evaluate the antibacterial four root extracts of this plant obtained by the method of successive solvent extraction with increasing polarity: dichloromethane, ethyl acetate, ethanol 96%, and water. It is performed on three reference strains including Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and six resistant clinical strains including Staphylococcus aureus methicillin-R (MRSA), Pseudomonas aeruginosa imipenem-Intermediate, Escherichia coli (ESBL), Salmonella Typhi (ESBL) Klebsiella pneumoniae (ESBL) and Enterobacter cloacae (ESBL).The method of wells in the agar was used to test the sensitivity of bacterial strains while the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the method of broth dilution. The dichloromethane and aqueous extracts did not give significant inhibition diameters. As against, the ethanolic and acétatic extracts gave inhibition diameters between 8 and 21 mm on the strains tested. Furthermore, the latter showed bactericidal power on all these tested strains and especially on S. aureus methicillin-R, Pseudomonas aeruginosa Imp-I, S tyhi (ESBL) with MIC and MBC ranging from 6.25 and 50 mg / mL. The phytochemical analysis of root extracts Cochlospermum planchonii showed the presence of polyphenols, flavonoids, tannins, alkaloids, sterols and carotenoids and polyterpenes. The sensitivity of the bacteria tested justifies the use of this plant in traditional community to combat diseases in which the tested bacteria are involved including infections associated with cough and diarrhea.
Authors and Affiliations
Yéo Sounta Oumar, Ouattara Karamoko, Konan K. Fernique, Djaman Allico Joseph, Dosso Mireille, Coulibaly Adama, Guessennd Kouadio Nathalie
Keywords
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