Comparative Evaluation of Cariogenic Potential of Natural and Unrefined Sweeteners on Streptococcus Mutans Biofilm Formation and Enamel Demineralization-In Vitro Study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 3
Abstract
Dental caries is the most common infectious diseases found in human beings.Controlling the frequency of intake of dietary sugar and use of alternative sugars in foods have therefore been recommended as the preventive strategy for public and private in dental caries management. For biofilm assessment Six 10% solutions containing xylitol, sucrose, honey, jaggery, palmsugar and stevia were prepared. MTT assay was used to evaluate microbiological counts in vitro.For enamel demineralization assessment, a total of 120 extracted premolars were immersed in six group of sugar solutions and 1.5 × 108 cells of Streptococcus mutans were inoculated into each group for 21 days. Buccolingual sections of teeth were evaluated at three points under a polarized microscope. Higher in vitro S. mutans biofilm formation and was observed in sucrose solution (p < 0.01).Least invitro biofilm formation and depth of enamel demineralization was found for xylitol followed by stevia,honey,jaggery,palmsugar and the highest was with sucrose group. Within the limitations of the present study, it may be concluded that even though xylitol group exhibited the least mean depth of enamel demineralization, it cannot be statistically proved better than the stevia and honey group.
Authors and Affiliations
Ambily Jayadevan, Dhanavel Chakravarthy, Padmaraj S. N, Vijayaraja S, Limly Bal, Nandini Dimple
Keywords
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- EP ID EP555724
- DOI 10.9790/0853-1803086370.
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