Comparative In Vitro Assessment of the Methanol Extracts of the Leaf, Stem, and Root Barks of Cnidoscolus aconitifolius on Lung and Breast Cancer Cell Lines
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 4
Abstract
Objectives: Cnidoscolus aconitifolius Mill. I.M.Johnst. is a medicinal plant widely used in ethnomedicine for the treatment of cancer and other diseases. Materials and Methods: The effects of methanol extracts of the leaf, stem, and root barks were evaluated on breast (MCF-7) and lung (NCI-H460) cancer cells at 1-250 μg/mL using the SRB assay and the extracts were screened for phytochemicals using the standard method. Results: The stem and root extracts showed no activity at the maximum concentration, while the leaf extract at 100 μg/mL showed remarkable cell growth inhibition against breast (-14.50±0.58) and lung cancer (+53.29±4.57) in vitro. The extracts showed the presence of saponins, terpenes, cardiac glycosides, and phenolic compounds. Partitioning of the active leaf extract further enhanced its activity as the chloroform fraction exhibited GI50, LC50, and total growth inhibition (TGI) of 22.5, 68.75, and 43.75 μg/mL against breast cancer, respectively, and GI50 and TGI of 35.4 and 55.8 μg/ mL against lung cancer cells, respectively. However, the aqueous fraction showed no cytotoxicity against either cell line. Conclusion: These results justified the ethnomedicinal uses of the plant against tumor-related ailments. Isolation of the constituents responsible for the observed activity needs to be carried out to further support this claim.
Authors and Affiliations
Emmanuel Oise OIKPEFAN, Buniyamin Adesina AYINDE, Azhar MUDASSIR, Ahsana Dar FAROOQ
Keywords
Related Articles
- EP ID EP670127
- DOI 10.4274/tjps.galenos.2018.19942
- Views 110
- Downloads 0
Formulation and Optimization of Gentamicin Hydrogel Infused with Tetracarpidium Conophorum Extract via a Central Composite Design for Topical Delivery
Objectives: Response surface methodology coupled with statistically designed experiments has been found to be very useful in optimising multivariable processes. The aim of this study was to evaluate the influence of two...
Use of Water Soluble and Phosphorescent MPA-capped CdTe Quantum Dots for the Detection of Urea
Objectives: To describe a method for the determination of urea in blood serum using urease enzyme and 3-MPA-capped CdTe quantum dots. Materials and Methods: The method is based on the increase in pH of the solution as a...
Development and Validation of a High-performance Liquid Chromatography–Diode-array Detection Method for the Determination of Eight Phenolic Constituents in Extracts of Different Wine Species
Objectives: A new HPLC method was developed and validated for the determination of some phenolic compounds; gallic acid, chlorogenic acid, epigallocatechin, caffeic acid, vanillin, p-coumaric acid, rutin, and quercetin i...
Quetiapine Fumarate Extended-release Tablet Formulation Design Using Artificial Neural Networks
Objectives: This design study was implemented within the scope of the quality by design approach, which included the “International Conference on Harmonization” guidelines. We evaluated the quality of a modified-release...
Melatonin in Edible and Non-Edible Plants
The concept of melatonin has become more important recently both in plants and in human who utilize plants for nutritional and health purposes. Melatonin, synthesized from L-tryptophan by enzyms, protects plants against...