Use of Water Soluble and Phosphorescent MPA-capped CdTe Quantum Dots for the Detection of Urea

Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2018, Vol 15, Issue 2

Abstract

Objectives: To describe a method for the determination of urea in blood serum using urease enzyme and 3-MPA-capped CdTe quantum dots. Materials and Methods: The method is based on the increase in pH of the solution as a result of the reaction between urea and urease, which causes an increase in the phosphorescence signal of MPA-CdTe quantum dots in the pH range of 2.5-5.0. Under the optimum conditions, the linear range of urea was 0.016-0.16 mM (1-10 mg/L) and the limit of detection based on 3 s/b was calculated as 0.003 mM (0.17 mg/L). The relative standard deviation was calculated as 3.4% at 4 mg/L urea concentration (n=7). Results: The method was applied to human serum samples. The same samples were analyzed by an independent laboratory and the results were not statistically different, at 95% confidence level (F test). Conclusion: The proposed method does not need sample pretreatment, is simple, selective, and cost-effective for the determination of urea in serum samples.

Authors and Affiliations

Tülay OYMAK, Nusret ERTAŞ, Uğur TAMER

Keywords

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  • EP ID EP382439
  • DOI 10.4274/tjps.02986
  • Views 153
  • Downloads 0

How To Cite

Tülay OYMAK, Nusret ERTAŞ, Uğur TAMER (2018). Use of Water Soluble and Phosphorescent MPA-capped CdTe Quantum Dots for the Detection of Urea. Turkish Journal of Pharmaceutical Sciences, 15(2), 44-49. https://europub.co.uk/articles/-A-382439