Comparative Molecular and Structural Characterisation of Chikungunya Virus Isolated Before and After 2006 Epidemics
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 2
Abstract
Background: Chikungunya virus caused incapacitating infection during 2006 epidemics spanning 28 countries. The expansion of the disease and increased virulence resulted in neurological and ocular complications in the affected patients implicating changes in the structural and functional properties of the newly emerging strains of Chikungunya virus. Objective: Hence the study was designed to understand the disparity between two strains isolated before and after 2006 epidemics by both in vitro and in silico approaches with respect to E1 gene. Materials and Methods: Sequencing of E1 gene and phylogenetic analysis of the two strains were carried out followed by the determination of growth pattern. The impact of aminoacid substitutions on the structural properties of E1 protein between the strains of Chikungunya virus was identified by different Bioinformatic tools. Results: Sequencing and Phylogenetic analysis revealed the two strains as Asian (isolated before 2006) and East Central South African (isolated after 2006). ECSA strain produced 1.5 fold log10 titre increased viral production than the Asian strain at the multiplicity of infection 1. Influence of aminoacid difference on the structure of E1 protein between two strains by Bioinformatic analysis had shown a change in the conformations by the loss of two intermolecular hydrogen bonds in 121 position in Asian strain and electrostatic differences in 211 positions between the two strains was also observed. Conclusion: As the interacting aminoacid 121 and 211 position lies near the fusion loop hence the difference in aminoacid position between the two strains could better influence fusogenicity and stable trimer formation. This forms a preliminary insight on the impact of aminoacid substitutions on the structural properties of E1 protein between the strains of Chikungunya virus and further experimental investigations are warranted.
Authors and Affiliations
K. Sangeetha, Indu Purushothaman, S. Rajarajan
Keywords
Related Articles
- EP ID EP350752
- DOI 10.9734/BMRJ/2016/27880
- Views 76
- Downloads 0
Prevalence of Canine Parvovirus in Jos North and South Local Government Areas of Plateau State
The research was conducted to determine the prevalence of canine parvovirus in dogs in Jos-North and South Local Government Areas of Plateau State. The prevalence in relation to sex, location, vaccination status, age and...
Field and In-vitro Studies on Corynespora Leaf Fall Disease of Hevea brasiliensis in Nigeria
Aims: This study evaluated the current incidence and severity of Corynespora leaf fall disease (CLFD) in the fields among cultivated rubber clones grown in Nigeria and the growth performance of selected isolates of the p...
Efficacy of Single Dose of 500 mg Mebendazole on Geohelminths amongst School Age Children in Bafoussam, Cameroon
Background: The aim of this study was to evaluate the efficacy of mebendazole in the treatment of Ascaris lumbricoides, Trichuris trichiura and hookworm infections amongst school age children in Bafoussam. Methodology: I...
Immobilization and Thermostability of Manganese Peroxidase from Trichoderma harzianum
The aim of the present investigation was to isolate Manganese peroxidase (MnP) (EC 1.11.1.13) from Trichoderma harzianum. The optimal pH and temperature for the enzyme production were 5.0 and 30°C. The best...
Suppression of Phytopathogenic Fungi by Plant Extract of Some Weeds and the Possible Mode of Action
Aims: The goal of this work was to emulate the antifungal properties of some newly collected weeds against important phytopathogenic fungi: Pythium ultimum, Penicillium expansum and Fusarium solani. The mode of action th...