Comparative study of enzyme linked immunosorbent assay and immunochromatography for rotavirus detection in children below five years with acute diarrhoea
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 4, Issue 3
Abstract
Background: Early diagnosis of rotavirus infection prevents unnecessary use of antibiotics, minimizes it’s spread and helps to determine the appropriate treatment. Rapid test using latex agglutination or lateral flow immunochromatography is a good alternative to EIA with good sensitivity. The present study was undertaken to compare rapid immunochromatography method with Enzyme linked immunosorbent assay (ELISA) for detection of rotavirus antigen in stool sample. Material and Methods: Two hundred stool samples fromhospitalized children less than 5 years of age with symptoms of acute gastroenteritis were tested by rapid SD Bioline test and ELISA Kit (PremierTMRotaclone®). Sensitivity and specificity was compared. Results: All 200 stool specimens were processed for rotavirus antigen by rapid test and ELISA, out of which 26% were positive by rapid test and 28% were positive by ELISA. Sensitivity and specificity of rapid kit was studied considering ELISA as a gold standard. Sensitivity of rapid test was 89.29% whereas specificity was 98.6%. Discussion: Its early bed side diagnosis can be done by rapid test (lateral flow immunochromatography) with high sensitivity (89.29%) and specificity (98.6%).
Authors and Affiliations
N Mehta, S Baveja
Keywords
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- EP ID EP487288
- DOI 10.26611/1008432
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