Utility of GeneXpert as a diagnostic tool for extra pulmonary tuberculosis
Journal Title: Medpulse International Journal of Microbiology - Year 2018, Vol 7, Issue 2
Abstract
Background: India has the highest tuberculosis (TB) burden with high rates of MDR TB. Conventional TB culture considered as the gold standard often leads to considerable delay in diagnosis. GeneXpert newer diagnostic modality for TB not only detects Mycobacterium tuberculosis but also rapidly detects rifampicin resistance. Additional studies from various patient population is needed to reinforce the need of GeneXpert for diagnosis of extra pulmonary TB (EPTB). Aims: To evaluate the utility of GeneXpert for the detection of Mycobacterium tuberculosis (MTB) in extrapulmonary specimens. Settings and Design: A randomized prospective study at a tertiary care hospital in Mumbai over a period of one year. Methods and Material: 100 suspected EPTB specimens [pus, tissue, pleural fluid, ascitic fluid, synovial fluid, cerebrospinal fluid (CSF) and urine] were subjected to AFB smear, culture by LJ and MGIT and GeneXpert as per standard protocols and results were compared. Statistical Analysis: Significance of differences among categorical variables was examined by the Chi-square test and Fischer’s exact test Results: AFB smear positivity was only 35%, while using GeneXpert, overall MTB detection rate and rifampicin resistance rate was 50% and 14% respectively. Sensitivity and specificity of GeneXpert were 89.58%, and 86.54% respectively with PPV- 86.00% and NPV- 90.00%. Conclusions: GeneXpert when compared with culture, shows high sensitivity and specificity for the diagnosis of EPTB.
Authors and Affiliations
Basu Shaoli, Baveja Sujata, Swami Anjali M, Taklikar Shripad
Keywords
Related Articles
- EP ID EP414093
- DOI -
- Views 72
- Downloads 0
Chlamydial infection association with syphilis in patients attending STD clinic in a tertiary care hospital
Background: Co-infection of Chlamydia with syphilis highlights the importance of early laboratory diagnosis and specific treatment. It is worthwhile to screen patients for this infection and to initiate prompt treatment...
Prevalence and antibiotic sensitivity of pathogens isolated in urinary tract infection in a medical college hospital
UTI is a common cause of morbidity worldwide. It is mostly caused by E coli or other gram negative bacteria. Many of the organisms have become resistant to common antibiotics, The present cross sectional study was conduc...
Characteristics of stool in rotavirus and non rotavirus diarrhea cases: A comparative study
Background: Diarrhea is a leading cause of child mortality worldwide. Rotavirus is one of the most common causes of severe diarrhea and dehydration in children. Naked eye examination of stool for the presence of abnormal...
Detection of prevalence of antinuclear antibodies in clinically suspected cases of systemic autoimmune diseases by indirect immunofluorescence test in a tertiary care hospital
Background: Systemic autoimmune diseases (SAD) are the diseases where multiple organs are involved in the presence of auto antibodies directed against sub-cellular structures or molecules and are characterized by presenc...
Speciation and resistotyping of coagulase negative staphylococci from clinical samples in a tertiary care hospital
Background: Coagulase negative Staphylococci (CONS) are the indigenous flora of the human skin and mucous membrane. They have long been considered as non-pathogenic and were rarely reported to cause severe infections. Ho...