Comparative study of two automated pre-transfusion testing systems (microplate and gel column methods) with standard tube technique
Journal Title: International Journal of Blood Transfusion and Immunohematology - Year 2017, Vol 7, Issue 1
Abstract
Aims: Clinically significant antibodies may become undetectable and still provoke transfusion reactions. Hemolysis has been reported among transfusion recipients with anti-Kidd that was undetectable by gel column but detectable by other methods. Methods: We compared two automated technologies - microplate (MP) and gel column (GC) methods - with manual methods in ABO/RhD typing, irregular antibody screening, identification, titration, and detection threshold of mixed-field agglutination. Results: Automated systems agreed generally with tube results in 98% or more of ABO forward and RhD groupings, but showed weaker reactions in ABO reverse testing against A1 (K=0.88 with MP and K=0.77 with GC) and B cells (K=0.66 with MP and K=0.68 with GC) and failed to detect some anti-A (2 of 273 samples with MP) and anti-B (2 of 273 with MP and 1 of 272 with GC). MP missed 2 (anti-E and –Fyb) of 8 antibodies and GC missed 5 (2 anti-E and 1 each of -Fyb, -Jka and –Lea) of 10 antibodies, which manual PEG-IAT detected. Among 11 known alloantibodies, MP detected 7 antibodies at higher dilution than tube PEG-IAT, whereas GC showed lower scores in 7 samples than tube PEG-IAT and missed 2 clinically significant antibodies (anti-C and anti-Fyb). Conclusion: Automated systems, comparable to manual tube technique for forward grouping of ABO and RhD, are less sensitive in ABO reverse testing. As GC more often failed to detect clinically significant antibodies of low-titer, users should be especially mindful of possible post-transfusion hemolysis.
Authors and Affiliations
Hitoshi Ohto, Takako Ono, Hiroyasu Yasuda, Rie Hikichi
Keywords
Related Articles
- EP ID EP209877
- DOI 10.5348/ijbti-2017-30-OA-3
- Views 128
- Downloads 0
Seroprevalence and risk factors for human T cell lymphotropic viruses types 1 and 2 among blood donors in Jos, Nigeria
Aims: To determine the seroprevalence and risk factors of human T cell Lymphotrophic viruses 1 and 2 among blood donors in Jos, Plateau state, Nigeria. Methods: A cross sectional study of 500 consecutive blood donors fr...
Induced pluripotent stem cells: An update
Introduction: The discovery that the mature cells can be reprogrammed to become pluripotent increased the enormous interest in induced pluripotent stem cell (iPSC) technology for their potential application in biomedical...
Iron deficiency in regular blood donors in Enugu (Southeastern Nigeria)
Aims: Regular donors are often given some sort of non-monetary recognition. In Nigeria, about 92.9% of individuals donate blood because of the benefits they get from hospital. The aim was to study iron status of regular...
A new look at an old case: An auto-anti-P with pseudo-LKE activity
Aims: LKE is a high-incidence, minor RBC glycosphingolipid, related to both Pk and P antigens. Approximately 1% individuals are LKE-negative. However, antibodies against LKE are rare, with only six cases mentioned in lit...
Lifetime risk and characterization of red blood cell alloimmunization in chronically transfused patients with sickle cell disease
Aims: Patients with sickle cell disease (SCD) are often exposed to multiple units of blood transfusions predisposing them to the development of alloantibodies. Alloantibodies make subsequent transfusion difficult, costly...