Comparison between Staphylococcus aureus, strains isolated from medical students in pre-clinical formation and clinical training
Journal Title: Revista Cuarzo - Year 2015, Vol 21, Issue 2
Abstract
OBJECTIVE: To establish the differences between Staphylococcus aureus isolates from medical students in pre-clinical and clinical tra-ining and identify the level of susceptibility to methicillin, vancomy-cin and alternative antibiotics. MATERIALS AND METHODS: A cross-sectional observational de-sign with non-random sampling was used in medical students during pre-clinical and clinical training. Samples were taken from nasal swabs and cultured on blood agar. For beta-hemolytic gram-positive cocci, catalase and coagulase tests were performed and then cultured on man-nitol salt agar. Susceptibility to cefoxitin, oxacillin, linezolid, clinda-mycin and trimethoprim sulfamethoxazole was assessed by using the Kirby-Bauer technique, and for vancomycin, an E-test was performed (Biomerieux®). RESULTS: 51 strains of S. aureus from nasal swabs were isolated from 112 medical students. 68.6% were identifi ed as methicillin-sensitive (MSSA) and 31.4% as methicillin-resistant (MRSA). Four MRSA strains showed vancomycin intermediate (VISA 4-8 μg/mL) profi le, 41% of MSSA isolates was resistant to clindamycin, 31% to linezolid and 23.5% to trimethoprim sulfamethoxazole. DISCUSSION: MSSA, MRSA and VISA strains are present in nos-trils of our medical students, with MRSA showing high resistance levels (>50%) to clindamycin, TMP-SMX and linezolid, and MSSA levels up to 40%. These fi ndings reiterate the need to accomplish good nasal and hands hygiene in order to minimize the spread of S. aureus in community and healthcare facilities
Authors and Affiliations
Africano, F. , MéndezI. , Holguin, D. , PachónD. , Rojas, N. , & Gonzalez, I.
Keywords
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- EP ID EP694591
- DOI https://doi.org/10.26752/cuarzo.v21.n2.36
- Views 174
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