Comparison of IgM enzyme linked immuno sorbent assay with Weil-felix agglutination test for diagnosis of Scrub typhus in South India
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 1
Abstract
Introduction Scrub typhus is a disease caused by Orientia Rickettsia tsutsugamushi which belongs to Rickettsiaeceae family Early diagnosis of Scrub typhus is important because timely antimicrobial therapy may help an excellent response and prevent complications Serology is widely used for diagnosis of scrub typhus The Aim of this study isto detect the IgM antibodies by Capture Enzyme linked immuno sorbent assay ELISA and Weil Felix agglutination test against Scrub typhus and to compare the results of IgM ELISA with Weil Felix testMaterials and MethodsTotal of 723 blood samples were collected from clinically suspected Scrub typhus patients IgM ELISA commercial and Weil Felix agglutination test for Scrub typhus were performed on all serum samplesResults Among 723 patients 3142 showed positive by WeilFelix test against OX K antigen and 43 6 showed positive by IgM ELISA Among 43samples which were positive by IgM ELISA 1318 showed negative by WeilFelix test Out of 31samples positive by WeilFelix test one sample showed negative by IgM ELISA The sensitivity of IgM ELISA and Weil Felix agglutination test is 9677 and 6976 respectively ConclusionEarly diagnosis and treatment will prevent complications of Scrub typhus This study has found out the IgM ELISA is highly sensitive and dependable for diagnosis of scrub typhus than Weil Felix agglutination testKeywords IgM Enzyme linked immuno sorbent assay Scrub typhus Weil Felix agglutination Test
Authors and Affiliations
Meerah Srinivasagam, Vijayalakshmi Arumugam, Rajeswari Jayakumar
Keywords
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- EP ID EP473840
- DOI 10.18231/2394-5478.2018.0019
- Views 66
- Downloads 0
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