Comparison of immunochromatogenic method of antigen detection and Giemsa staining with polymerase chain reaction (PCR) for detection of chlamydia trachomatis infection in infertile women
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 4, Issue 1
Abstract
Context: Chlamydial infection is a silent epidemic. The varied prevalence of chlamydial infection in India, leads to an urgent need to look for simple, inexpensive and sensitive tests for early diagnosis. Aims: To determine the utility of a rapid Chlamydia antigen detection test and Giemsa staining for screening. Settings and design: Prospective cross-sectional hospital based study, approved by Institutional Ethics Committee. Material and methods: Three endocervical specimens were collected aseptically from 119 women aged 18-45 years. Of these, 89 patients presenting with primary and secondary infertility were taken as study group and 30 healthy term pregnant women as control group. Rapid antigen detection test and Giemsa stain were done as per standard protocol. PCR was the gold standard for comparison. Result: Out of 119 subjects, one infertile case was positive for C. trachomatis infection by rapidantigen detection test and was confirmed by PCR and none was positive by Giemsa test. Sensitivity and specificity of rapid test was 100% with100% positive and negative predictive value on comparison with PCR. For Giemsa staining specificity was100% with a 99.16% negative predictive value. Conclusion: In the absence of facilities for gold standard tests, screening patients with an efficient rapid antigen detection testcan play a significant role in screening of C. trachomatis and should beincorporated in routine infertility investigations.
Authors and Affiliations
K M Lohi, C A Kumar, J M Pramanik, S M Baveja
Keywords
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- EP ID EP487285
- DOI 10.26611/1008412
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Comparison of immunochromatogenic method of antigen detection and Giemsa staining with polymerase chain reaction (PCR) for detection of chlamydia trachomatis infection in infertile women
Context: Chlamydial infection is a silent epidemic. The varied prevalence of chlamydial infection in India, leads to an urgent need to look for simple, inexpensive and sensitive tests for early diagnosis. Aims: To determ...