Comparison of Mec A Gene in Staphylococcus Aureus Isolated from Children at Time of Admission and Discharge in West of Iran
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 5
Abstract
Staphylococcus aureus (SA) is a species of the genus Staphylococcus that is found everywhere, including in the respiratory tract and on the skin of many adults and children, and that is considered one of the main pathogens in nosocomial and community acquired infections. The number of Methicillin-Resistant Staphylococcus aureus (MRSA) strains has increased globally over the past two decades. Determining the frequency of mecA gene in SA that represent MRSA in hospitalized children and comparing it upon hospital admission and discharge therefore appear essential. The present cross sectional study was conducted in children hospitalized in Imam Reza hospital in Kermanshah, Iran in 2012. Nasal specimens were collected from the 500 children at time of admission, considered as community acquired Staph aureus and those whose first nasal culture was negative were included for second culture at time of discharge, considered as hospital acquired Staph aureus. After identifying Staphylococcus aureus by common laboratory tests, methicillin resistance was determined using Oxacillin screening plates and mec -A gene. The prevalence of community acquired Staph aureus nasal carriers was estimated as 4.6% and the prevalence of its hospital-acquired type as 5.6%. The prevalence of mecA gene was 17/4% and 37% in two groups, suggesting no statistically significant differences between the two groups. The resistance to Erythromycin, Cefoxitin, Mupirocin and Clindamycin was 30/4%, 8/7%, 4/3% and 30/4% in the admission group and 40/70%, 7/4%, 7/4% and 18/5% in the discharge group, suggesting no statistically significant differences between the two groups. All the samples were sensitive to Vancomycin and Linezolid and no instances of resistance to these antibiotics were observed. The increase in the prevalence of MRSA is caused by the overuse of antibiotics, which facilitates the colonization of MRSA. Moreover, the increase in the resistance to commonly-used antibiotics is a warning for a more reasonable prescription of sensitive antibiotics such as Vancomycin, so as to prevent the emergence of multidrug resistant MRSA.
Authors and Affiliations
Keyghobad Ghadiri| Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, Baharak Novroozi| Microbiology MS, Kermanshah University of Medical Sciences, Kermanshah, Iran, Mansour Rezaee| Department of Biostatistics and Epidemiology, Kermanshah University of Medical Sciences, Kermanshah, Iran, Lida Khodadadi| Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, Parviz Mohajeri| Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, Shahla Masoomshahi| Nursing BS, Kermanshah University of Medical Sciences, Kermanshah, Iran, Soodeh Shahsavari| Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, Mandana Afsharian| Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, Zahra Roshanzamir| Markaz tebi Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Email: biostatistics.ssh@gmail.com
Keywords
Related Articles
- EP ID EP11667
- DOI -
- Views 214
- Downloads 9
Comparative efficacy of MIRU-VNTR and IS 6110-RFLP for differentiation of Mycobacterium tuberculosis isolates
The aim of this study was To characterize Mycobacterium tuberculosis isolates by MIRU-VNTR typing and IS 6110 – RFLP and to compare the efficiency of MIRU-VNTR typing and IS 6110 – RFLP in discriminating Mycobacter...
Treatment of Oral Soft Tissue Lesions and Wounds Using Nano-Antioxidants Infused Gel: A Case Report
It is a high functional gel containing vitamin C, E and Propolis with a nanoemulsion state. We reported to treat patients who had poorly-healing lesion or large wound defect after minor surgery or ulcer-like lesion by u...
THE USE OF PERITONEAL DIALYSIS IN THE MANAGEMENT OF PATIENTS WITH RENAL FAILURE AT INSTITUTE OF KIDNEY DISEASES, PESHAWAR
Peritoneal dialysis (PD) using an ordinary stylet cannula was studied in 253 patients (67% male and 33% female with age ranging from 3-67 years) suffering from renal failure. The study was conducted between January 2007...
SPONTANEOUS FUNDAL UTERINE RUPTURE IN A GRANDMULTIPARA; AN UNUSUAL SITE OF RUPTURE
Uterine rupture is an obstetric emergency and a catastrophe with attendant maternal and fetal morbidity and mortality. It is not uncommon in unscarred uterus, but commonly occurs in uterus with one or more previous scar...
Assessment of disaster planning in humanitarian supply chain management (HSCM), Khuzestan: 2012
Background and Objective: Disasters are scarce events; nevertheless, to prepare organizations for events and return to normal condition the accurate planning and education is essential. This study aimed to assess disast...