Correlation of Multidrug Resistance and Plasmid Profile in Extended Spectrum Beta Lactamase Producing Escherichia coli Isolated from Patients Suspected of Urinary Tract Infection
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 11, Issue 8
Abstract
Aims: To determine the prevalence of extended spectrum beta lactamase producing Escherichia coli (ESBL E. coli) strains among the total isolates and study the association between the antibiotic resistance and plasmid profiles of the isolates. Methodology: A total of 1258 urine samples were collected. Identification of Bacterial isolates was done using standard biochemical tests, antibiotic susceptibility pattern was determined using the Kirby - Bauer’s disk diffusion method and confirmation of the ESBL E. coli was done following CLSI guidelines. Isolation of Plasmid DNA of ESBL positive strains was done by alkaline lysis method. Results: Out of 303 isolates, 198 were E. coli. The isolates were tested for antibiotic sensitivity, MDR, ESBL and plasmid profiles. 59.09% of the E. coli isolates exhibited multi-drug resistance. 41(58.57%) out of 76 ESBL E. coli isolates possessed plasmids. Few isolates possessed single plasmid while other had multiple plasmids with different size ranged from 1 kb to 10 kb. Conclusions: High prevalence of ESBL E. coli was found with good association between the antibiotic resistance and plasmid profiles of the isolates.
Authors and Affiliations
Vivek Kumar Singh, Mahesh Kumar Chaudhary, Reshma Tuladhar
Keywords
Related Articles
- EP ID EP340165
- DOI 10.9734/BJMMR/2016/21451
- Views 66
- Downloads 0
Audiometric and Tympanometric Assessment in Patients with Oral Submucous Fibrosis
Background: Oral submucous fibrosis (OSMF) is regarded as a potentially malignant condition. It is characterized by a mucosal rigidity of variable intensity because of the fibroelastic changes of the juxta epithelial lay...
A Study of Malnutrition-dependent Factors among Under-five Children in Ekureku Community, Abi Local Government Area of Cross River State, Nigeria
Malnutrition is the consumption of dietary nutrients either insufficiently or exclusively, and several socio-economic, ignorance, and educational factors are known to determine nutritional status of any community. This s...
Outcomes of Acute Kidney Injury Patients with and Without Cancer: A Single Center Study
Background: Acute kidney injury (AKI) is a frequent complication in hospitalized patients. Incidence of AKI in hospitalized patients with cancer is increasing, but there have been few studies on AKI in patients with canc...
The Multifaceted Effects of Vitamin D and Its Potential Contribution to Rheumatoid Arthritis
Vitamin D (VD) is known to have pleiotropic effects on various tissues and organs including those of the immune and musculoskeletal systems. VD deficiency has been suggested to trigger the onset of autoimmune diseases in...
Comparison of N-terminal Pro-B type Natriuretic Peptide and Tissue Doppler in Diagnosing Diastolic Heart Failure
Background: Heart failure (HF) has become a main cardiac problem. Doppler echocardiography has been used to examine left ventricular (LV) diastolic filling dynamics. Limitations of this modality suggest the need for othe...