Could rapidly mutating Y-STRs be a potential forensic tool in discriminating Lebanese monozygotic twins?
Journal Title: Genetics & Applications - Year 2018, Vol 2, Issue 2
Abstract
Despite the high power of discrimination that characterizes the well identified 16 to 24 autosomal short tandem repeat markers, monozygotic twins differentiation is generally limited. Arising from a single fertilized egg, monozygotic twins share the same genotype therefore the same DNA profile. This situation imposes a challenge in forensics especially considering that lineage markers are in general less informative than autosomal ones. Although in some cases Y haplotype is considered a powerful investigative tool, it cannot distinguish males belonging to the same paternal lineage. The use of rapidly mutating Y-STRs with mutation rate above 1x10-2 that were recently included in forensic casework is presumed helpful. Since science is always dynamic and each population has its own characteristics, we aim in this study to distinguish between Lebanese monozygotic male twins using rapidly mutating Y-STRs. For this purpose, fourteen unrelated pairs of male monozygotic twins were recruited. Participants filled in a well-designed questionnaire and signed an informed consent. DNA was extracted using PureLink Genomic DNA Mini kit, genotyped using the Identifiler Plus kit, and separated on 3500 Genetic Analyzer to confirm the monozygosity status. DNA samples underwent a second amplification using the Y Filer Plus kit. According to our results, all the Y Filer Plus DNA profiles showed complete match for each twin pair. By consequence, the use of rapidly mutating Y-STRs in this study did not improve discrimination.
Authors and Affiliations
Paula Romanos, Jamilah Borjac
Keywords
Related Articles
- EP ID EP520636
- DOI 10.31383/ga.vol2iss2pp35-42
- Views 91
- Downloads 0
AIR POLLUTION TOLERANCE INDEX OF Plantago major IN STEELWORKS AREA OF ZENICA, BOSNIA AND HERZEGOVINA
Atmospheric pollution is among the largest anthropogenic impacts on the ecosystem. In numerous studies it was observed that plants, especially those that grow in urban areas, are heavily influenced by different pollutant...
KNOWLEDGE OF ELEMENTARY GENETIC TERMINOLOGY AMONG HIGH SCHOOL STUDENTS IN BOSNIA AND HERZEGOVINA
Genetics, as a science of the future, is very important part of Biology teaching. Within educational system in Bosnia and Herzegovina it is studied in limited number of clasess with a minimum of practical excercises, in...
Role of host and viral factors and genetic variation of IL28B on therapy outcome in patients with chronic hepatitis C genotype 1b from Serbia
Viral and host factors in hepatitis C virus (HCV) infection can influence on therapy outcome to pegylated interferon/ribavirin (PEG-IFN/RBV) and progression of liver fibrosis. Although novel direct-acting antivirals (DAA...
RELATIVE GENE EXPRESSION ANALYSIS FROM URINE SAMPLES OF PATIENTS WITH PROSTATE DISEASE
Conventional screening and diagnostic procedures are leaned on PSA (Prostate specific antigene) which is not specific for prostate cancer and frequently leads to unnecessary invasive procedures for the exclusion of malig...
INGDIF - SIMPLE MEASURE BASED ON ANALYSIS OF GENETIC DIFFERENTIATION
We suggest simple measure based on the analyses of genetic differentiation. The purpose of this measurement is to estimate deviation of each locus FST (GST, θ) from overall value. This index of genetic differentiation (I...