Cross-contamination in routine diagnostic in the mycobacteriology laboratories – risk factors, detection and prevention
Journal Title: Postępy Nauk Medycznych - Year 2015, Vol 28, Issue 4
Abstract
Introduction. Identification of Mycobacterium tuberculosis complex in clinical material from the patient is the basis of microbiological confirmation of TB, and remains gold standard for diagnosis. The results of numerous studies show that about 0.1-4% derived culture may be the result of cross-contamination. It occurs most frequently during processes of collection and processing of clinical samples, as a result of infection during medical procedures.Aim. Determining the incidence of cross-contamination in the National Tuberculosis Reference Laboratory during 2011-June 2014.Material and methods. The material for the study were 598 isolates of Mycobacterium tuberculosis complex from 408 patients with suspected tuberculosis. For the epidemiological and molecular analysis we selected 26 (4.3%) cultures, which, according to the CDC guidelines, could be potentially false-positive. The information that determined the choice of strains is the same date of inoculation and the sequence number of the study or in close proximity to the material from which the culture was obtained. Molecular analysis of the strains was performed using spoligotyping and MIRU-VNTR methods.Results. We examined 12 potential cross-contamination incidents. Finally, contamination was confirmed in 4 cases. The consequence was to obtain 5 (0.8%) false-positive cultures of M. tuberculosis complex.Conclusions. Detection of cross-contamination of materials from patients with suspected tuberculosis has epidemiological, clinical and therapeutic significance. Proper system of monitoring and identification of false positive cultures requires reliable medical documentation and using appropriate genotyping methods.
Authors and Affiliations
Monika Kozińska, Katarzyna Wasiak, Magdalena Klatt, Ewa Augustynowicz-Kopeć
Keywords
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