CYTOTOXICITY AND APOPTOTIC INDUCIBILITY OF HYDRO-ETHANOLIC EXTRACT OF AERIAL PARTS OF POTHOS SCANDENS L. AGAINST MCF-7 BREAST CANCER CELL LINE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: To investigate the ability of 50% hydro-ethanolic extract of Pothos scandens (P. scandens) to induce cytotoxicity and apoptosis in MCF-7 cell lines.Methods: Aerial parts of P. scandens were extracted with 50% ethanol. The crude extract obtained was evaluated for its ability to induce cytotoxicity against MCF-7 (breast cancer) and L929 (normal fibroblast) cell lines by MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay. The ability to induce apoptosis was determined by acridine orange (AO) and ethidium bromide (EB) double staining method. The above activity was confirmed using the comet assay.Results: The study revealed that the plant extract showed significant cytotoxic activity against MCF-7 cell lines with an IC50 of 90.18±5.20 µg/ml. Further it was also found that the cell death of MCF-7 treated with crude extract was due to the induction of apoptosis, which was ascertained by comet assay.Conclusion: The study provides scientific evidence for the anticancer activity of extracts of P. scandens paving a way to further research of using this plant in the development of the novel anticancer drug.Keywords: P. scandens, MCF-7, L929, MTT assay, Apoptosis, Comet assay
Authors and Affiliations
Jethinlalkhosh J. P. , Sarath S Nair, Praveena P. , Victor Arokia Doss D
Keywords
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