DESIGN OF HYDROQUINONE INDICATOR STRIP BASED ON POLYMETHYLMETHACRYLATE (PMMA) FOR IDENTIFICATION OF HYDROQUINONE IN WHITENING COSMETICS: CASE STUDY IN BANDUNG INDONESIA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: The objective of this research was to developing an indicator strips made from polymer polymethylmethacrylate (PMMA) to detect hydroquinone in whitening cosmetics.Methods: The strips were prepared by reagent blending method with a polymer PMMA that mixed with a chemical reagent for hydroquinone based on chemical structure: ferric chloride (FeCl3), Benedict, and ammoniacal silver nitrate. PMMA were varied with concentrations of 5%, 7.5%, and 10%. The ratios of ethyl acetate and the reagents were off 6:4; 7:3; and 8:2.Results: The results showed that the following concentration was an optimum mixture of chemical reagent-polymer and ethyl acetate FeCl3-PMMA 7.5% 7:3; benedict-PMMA 5% 6:4; and ammoniacal silver nitrate-PMMA 5% 7:3. 22 samples of whitening cream were not contained hydroquinone based on strip indicator analysis. These results are appropriate with a confirmatory test using UV spectrophotometry and thin layer chromatography. The indicator strips showed a positive result in detect the samples that had been spiked by hydroquinone but has a limitation when applied to cream dosage forms. The limit of detection (LOD) and stability of the indicator strip FeCl3-PMMA 7.5% 7:3 was 20.05 mg/l and stable up to 157 d, Benedict-PMMA 5% 6: 4 was 0177 mg/l and stable up to 123 d, and ammoniacal silver nitrate-PMMA 5% 7:3 was 0.025 mg/l and stable up to 174 d. The selectivity test results showed that indicator strip did not give false positive results.Conclusion: Based on the tests, the indicator strip has limitations to detect hydroquinone in whitening cream cosmetics that required further modifications to the detection process.Keywords: Hydroquinone, UV-Vis spectrophotometry, Thin Layer Chromatography (TLC), Indicator strip, Polymethylmethacrylate (PMMA)
Authors and Affiliations
Yunita Kurniati, Anisa Desy Aryanti, Danni Ramdhani, Driyanti Rahayu, Mutakin . , Aliya Nur Hasanah
Keywords
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