Detection of cryptosporidium in fecal sample by three staining method from immunocompromised patients

Abstract

Introduction Cryptosporidium species is the most common opportunistic enteric parasite encountered in the immunocompromised patientsObjective To evaluate Modified ZiehlNeelsen staining auramine phenol staining and safranine methylene blue for the diagnosis of intestinal cryptosporidiosisMaterials and Methods A total of 70 faecal samples from HIV seropositive were collected They were subjected for modified ZN stain safraninemethylene blue stain and auramine phenol stain for detection of cryptosporidiumResults Out of total 70 HIV patients 57 81 were positive for parasitic infection higher number of parasitic infection is caused by Cryptosporidia in 34 60 Of the 34 patients who had cryptosporidiosis were positive for auramine phenol technique 3397 of them were also positive with the modified ZN stain and 31910 were also positive with the safranine methylene blue stainConclusion Auramine phenol staining is a rapid sensitive and specific technique for diagnosis of intestinal cryptosporidiosisKeywords Cryptosporidium Modified ZN stain Safranine methylene blue stain Auramine phenol stain

Authors and Affiliations

Arti B. Ninama

Keywords

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  • EP ID EP485889
  • DOI 10.18231/2581-4761.2018.0055
  • Views 85
  • Downloads 0

How To Cite

Arti B. Ninama (2018). Detection of cryptosporidium in fecal sample by three staining method from immunocompromised patients. IP International Journal of Medical Microbiology and Tropical Diseases, 4(4), 257-260. https://europub.co.uk/articles/-A-485889