Detection of efflux pump in multidrug resistant acinetobacter Species using Naphthyl-Methyl-Piperazine
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 4
Abstract
Acinetobacter Gram negative bacterium has proven to be recently important to research due to multidrug resistance Multidrug resistance is directed against antibiotics like Aminoglycosides Carbapenems Cephalosporins Quinolones etc The presence of efflux pump primarily the ResistanceNodulationCell Division RND type is an active transport mechanism which allows it to efficiently reduce antibiotic levels within the cell 11naphtylmethylpiperazine NMP is an efflux pump inhibitor EPI which mechanically inhibits the efflux pump in bacteria resulting in increased concentration of antibiotic intracellularly thereby killing the cellObjectives To detect the efflux pumps phenotypically using NMP and to establish the cutoff zone diameter to detect efflux pump in Acinetobacter speciesMaterials and Methods Acinetobacter species were isolated from patient samples using conventional techniques of inoculation identification and antibiotic susceptibility testing The efflux pump was detected phenotypically by disc diffusion method on Mueller Hinton agar with NMP and chloramphenicol tetracycline discs as substrates The zone diameters were recorded values compared using ttest Tipping analysis was done to determine the mean zone difference to identify the presence of efflux pumpResults and Conclusion 61 of the Acinetobacter species were multidrug resistant of which 63 had efflux pump as detected by chloramphenicol substrate which is better than Tetracycline pKeywords Efflux pump Acinetobacter NaphthylMethylPiperazine
Authors and Affiliations
Sandeep T. , Indumathi V. A. , Prasanthi S.
Keywords
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- EP ID EP485882
- DOI 10.18231/2581-4761.2018.0049
- Views 76
- Downloads 0
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