Detection of Single Point Mutation of Plasmodium Falciparum Multi-Drug Resistance 1 Gene in Three Different Areas in Sudan
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 11
Abstract
Background: Chloroquine resistance in Sudan lead the ministry of health to change the first line of malaria treatment from chloroquine to artemisinin, this study was designed to detect single point mutation of Plasmdium falciparum multi-drug resistance-1 (Pfmdr-1) N86Y mutant allele in three different areas in Sudan. Materials and Methods: Three hundred (300) P.falciparum positive samples were collected from three areas in Sudan, positive samples were confirmed by using direct microscopical stained blood films, DNA was extracted using Chelix method and then were amplified using Nested RFLP-PCR method to detect the Pfmdr-1 N86Y mutant allele. Data were analyzed using SPSS 16.5 by Chi-squire test. Results: Single point mutation of Pfmdr-1 N86Y mutant allele was detected in the study areas including Khartoum (15.7%) , River Nile (10.7%) and Sennar (9.7%) respectively, Pfmdr-1 N86Y among the males and females were equal (18 %) which found to be statistically insignificant at P. value=0.167, finally the Pfmdr-1 N86Y in association with age groups, showed (8.0%, 4.7%, 15.7% and 7,7%) among age groups (<10, 10-19, 20-50 and >50) respectively which found to be statistically insignificant at P.value=0.399.
Authors and Affiliations
Ahmed Bakheet Abd Alla, Tayseer Elamin Mohamed Elfaki, Mohamed Baha Eldin Ahmed Saad, Ali Elamin Nasir, Ezzalarab Kamil Ahmed
Keywords
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- EP ID EP238870
- DOI 10.9790/0853-1611078994
- Views 138
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