Determination of the residual amount of aminoglycosides in poultry products by microbiological method

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Determination of the residual amount of aminoglycosides in poultry products by microbiological method

Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue

Abstract

Introduction. An urgent problem today remains the effective control of poultry products for the residual amounts of aminoglycosides, as they able to get through the food chain to the human body, and cause overgrowth, toxemia, carcinogenia, teratogenia, mutagenia allergia allergic reactions, secondary fungal infections, violation of mineral metabolism. They characterize as osteotropic and promote the development of antibiotic-resistant microorganisms in humans.The goal of the work was to conduct comparison of the national and European microbiological screening methods for antibiotic residual detection in poultry meat and eggs, and testing and validation of qualitative microbiological “NAT-screening” method of determination of aminoglycosides in poultry, eggs and egg products.Materials and methods. The sensitivity and specificity limits were established by the microbiological method “NAT-screening” to determine the residues of aminoglycosides. The study was carried out in 20 repetitions on simulated samples of poultry meat, eggs and egg products that are free from antibiotics, and also in samples with introduced antibiotic's standards in concentrations of ½ MRL and MRL.Results were treated by variation statistics methods using “Microsoft Excel - 15.0” software with calculating the arithmetic mean (M), standard deviation (m) and the level of probability (p) by Student's table. We determined the accuracy, specificity, sensitivity of the method.Results of research and discussion. We found clearly defined growth inhibition zone of pure test culture B.subtilis ATCC 6633, which ranged from 17.01±0.01 mm to 20.04±0.03 mm. The result of the study of microbiological screening method “NAT-screening” of low detection (½ MRL) of aminoglycosides residual amounts for meat 50 µg/kg for eggs and egg products - 250 µg/kg, corresponded of 100% specificity, accuracy and sensitivity.Conclusions and prospects for further research. Established that sensitivity of European microbiological method “NAT-screening” for residual aminoglycosides in meat was for paramomycin - 250 µg/kg, DHstreptomycin - 250 µg/kg, neomycin - 250 µg/kg, kanamycin - 50 µg/kg; for eggs and egg products - neomycin - 250 µg/kg corresponded MDR European law and order №695 from 06.08.2013

Authors and Affiliations

Ilona Azyrkina

Keywords

test culture Bac.subtilis ATCC 6633 paromomycin DHstreptomycin neomycin kanamycin eggs egg products NAT-screening.

EP ID EP266763
DOI 10.31073/vet_biotech30-01
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