Development and optimization of Etoposide loaded nanoparticles by using DoE response surface central composite design
Journal Title: International Journal of Bio-Pharma Research - Year 2019, Vol 8, Issue 4
Abstract
Cancer is a leading cause of death globally. The World Health Organizationestimates that 9.6 million people died of cancer in 2018. More than 70% of all cancer deaths occur in low- and middle-income countries, where resources available for prevention, diagnosis and treatment of cancer are limited or nonexistent. Present investigation aimed at formulation and optimization of nanoparticles with Etoposide, an anticancer drug. Design Expert software is used for optimization. A randomised response surface design was used for optimization procedure. Stirring speed and crosslinking agent concentration are the independent variables for the dependant variables of particle size and drug loading. Statistical data of the dependent variables obtained were subjected to the analysis of variance and were found significant at p<0.001 for particle size and at p<0.0001 for drug loading. Encapsulation efficiency of the NPs was evaluated and they were found to be between 65.87±2.78 - 88.95±1.09. The prepared NPs are with the minimum size of 98±8.76nm and a maximum size of 786.24±6.89nm. Through factorial design, it was optimised that 500rpm (at a level of 1) and the maximum concentration of glutaraldehyde at 25% (at a level of 1) will result a least mean particle size of 150.13nm and maximum drug loading of 86.24%.
Authors and Affiliations
Ramalingam Ramani, Bhrugun Anisetti
Keywords
Related Articles
- EP ID EP535002
- DOI 10.21746/aps.2018.8.4.2
- Views 181
- Downloads 0
Development and optimization of Etoposide loaded nanoparticles by using DoE response surface central composite design
Cancer is a leading cause of death globally. The World Health Organizationestimates that 9.6 million people died of cancer in 2018. More than 70% of all cancer deaths occur in low- and middle-income countries, where res...
A Comprehensive qualitative phytochemical estimation of Euphorbia neriifolia Linn.
Euphorbia neriifolia Linn. is a restorative plant of Euphorbiaceae family, utilized as Indian conventional medication in bronchitis, tumors, leucoderma, heaps, irritation, spleen broadening, weakness, ulcers, and fever....
Morpho-Anatomical studies of Atalantia racemosa Wight ex Hook., an important medicinal plant of Chittoor Dt. of Andhra Pradesh, India.
Atalantia racemosa Wight ex Hook., belongs to the family Rutaceae and is widely used as ethnomedicine in and around chittoor district of Andhra Pradesh for various ailments. In this paper we attempted for authenticity in...
Bioactive potential of Pseudomonas alcaliphila isolated from a marine sponge against human pathogens
Metabolite extraction is considered as one of the important steps in metabolomics, the marine metabolite are the new source of the most antimicrobial agents used in both pharmacological and biological applications. In th...
Development and Biopharmaceutical Characterization of BCS Class II Drug – Naproxen by Two Way Complexation Solid Dispersion Technique
The objective of this study was to increase the solubility and bioavailability of Naproxen (NP) by fabricating ternary solid dispersion (tSDs) with water soluble polymer PEG 6000 and crospovidone. tSDs were prepared and...