DEVELOPMENT AND USING OF MEASURING-CALCULATING COMPUTERIZING COMPLEX FOR REGISTRATION OF COLONO-VOLTAGE CHARACTERISTICS AND BIOSENSORY INDICATION OF PATHOGENS
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2014, Vol 11, Issue 1
Abstract
A fast measuring computerized system for registering culon-voltage characteristics (СVC) for interphase boundaries was created and approved. The indication dimensional phase (IDP) synthesized from antibodies to S. aureus on the metal surface of the indifferent electrode, where IDP have phase with nanoscale electrochemical reaction coordinate. It is shown that all the measured phase boundary СVC Pt | 1NANO MI antibody S. aureus | pathogenic S. aureus cells in PBS, located inside СVC from the interface Pt | 1NANO MI antibody S. aureus | PBS. The analysis of cullon-voltage characteristics carried out. Biosensor was designed and the electrochemical method for laboratory registration of pathogenic microorganisms strains was developed. The quantitative cullon-voltage method detection for immune complex (IC), that strongly adsorbed and associated with the biosensor working surface was developed. The IC formation is due to the complementary interactions of pathogenic cells - antibody. In this paper it was proposed to analyze the experimental data in logarithmic coordinates lg cS. aureus, theor = f(lg cS. aureus, exp) unlike widely used coordinate system cS. aureus, theor, c S. aureus, exp. It is shown that the basic patterns of biosensors, between the calculated and specified concentrations of pathogens, have correlation coefficients of 0.99 and above
Authors and Affiliations
V. V. Jelali, D. N. Chernyshenko, I. Y. Kuchma, N. A. Korotkova, T. P. Osolodchenko
Keywords
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- EP ID EP399357
- DOI 10.18524/1815-7459.2014.1.108138
- Views 81
- Downloads 0
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