DEVELOPMENT AND VALIDATION OF A DISSOLUTION METHOD FOR FROVATRIPTAN TABLETS BY REVERSED PHASE UPLC
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 4
Abstract
Objective: The main objective of the method was to develop a simple, rapid, efficient and reproducible, stability indicating reverse phase ultra performance liquid chromatography (RP-UPLC) method for the estimation of frovatriptan in tablet dosage form.Methods: The RP-UPLC method for estimation of frovatriptan (FRT) in their tablets was carried out on Acquity UPLCTM, BEH C-18 (100 × 2.1 mm, 1.7 µm) column using 0.1% trifluroacetic acid buffer and a mixture of methanol and acetonitrile (50:50) using isocratic program. The flow rate of the mobile phase was 0.2 mL min-1and detection wavelength was carried out at 244 nm. Total runtime is 3 minutes for chromatographic run. The method was validated in terms of specificity, linearity, accuracy, precision and robustness as per ICH guidelines.Results: The method was found to be linear in the range of 1.41-3.67 μg mL-1. Recovery was found to be in the range of 97.8-101.8%. Relative standard deviation for precision and intermediate precision was found to be less than 3%. The developed method was successfully applied for the estimation of frovatriptan in tablet formulation and average dissolution rate was found to be 93%. The results obtained from the validation experiments prove that the developed method is suitable for routine analysis.Conclusion: The developed RP-UPLC method was simple, rapid, accurate, and precise for the estimation of dissolution rate in frovatriptan tablet dosage form.Â
Authors and Affiliations
V. Ashok Chakravarthy, B. B. V. Sailaja, A. Praveen Kumar
Keywords
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