Development and Validation of A RP-HPLC Method For Estimation of Flunitrazepam In A Tablet Dosage Form
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 3
Abstract
A simple, sensitive, and precise high performance liquid chromatographic method for the analysis of Flunitrazepamhas been developed and validated for the determination of compound in commercial pharmaceutical products. The compounds were well separated on BDS Hypersil C18 reverse phase column by the use of a mobile phase of mixed phosphate buffer and acetonitrile in a ratio of 40:60 v/v, at a flow rate of 1.0 ml/min with detection wavelength at 248nm. The method was validated in terms of linearity, precision, accuracy, and specificity, robustness and solution stability. The method does require only 10 minutes as runtime for analysis which prove the adoptability of the method for the routine quality control analysis of the drug.
Authors and Affiliations
Debasis Bora. , Prof(Dr) S K Gupta
Keywords
Related Articles
- EP ID EP395178
- DOI 10.9790/3008-1303031216
- Views 93
- Downloads 0
Anti –Mullerian Hormone is a new Marker for Ovarian function in females with polycystic ovary syndrome
Anti-Mullerian Hormone (AMH) is a member of the transforming growth and differentiation factors in the ovary, AMH has an inhibitory effect on primordial follicle recruitment as well as on the responsiveness of growth fol...
A study on physico-biochemical efficiency of LBG 17 a Blackgram (Vigna mungo L. Hepper) genotype against powdery mildew
LBG 17 is a resistant inbred of Blackgram developed from susceptible parents Nethiminumu and chikkuduminumu to defend the damage due to powdery mildew. In present study the physiological efficiency of LBG 17, Nethiminumu...
Effect of Methanolic Extract of Emilia Praetemissa on Paracetamol Induced Liver Damage in Albino Wistar Rats
The present investigation shows the hepato-protective role of Emilia praetermissa in paracetamolinduced liver toxicity. The study was conducted using forty Albino rats randomly divided into four groups of ten rats each....
Evaluate the action of the aqueous extract of the medicinal plant Goji Berry (Lyciumbarbarum) on biochemical and hematological parameters in Wistar rats
Medicinal plants were discovered by man through the food demand, and since then, are used empirically for the treatment of conditions, or in the form of infusion, decoction or another, with the purpose to cure or at leas...
Alternative To Reduce Occupational Hazards For Paramedical Staffs In Histopathology Department
Introduction: Xylene (Xy) is an aromatic hydrocarbon known for its wide usage in tissue processing on human anatomical samples which has numerous health hazards. Objective: Use of edible oil such as coconut oil (Co) and...