DEVELOPMENT AND VALIDATION OF A RP- UFLC METHOD FOR ESTIMATION OF SYNTHETIC STEROID HORMONE DANAZOL
Journal Title: Hygeia:journal for drugs and medicines - Year 2016, Vol 8, Issue 1
Abstract
Plan: Danazol is a synthetic steroid hormone with anti-estrogenic and anti-gonadotropic activities drug which acts on anterior pituitary suppressant by inhibiting the pituitary output of gonadotropins indicated for the treatment of endometriosis and benign breast disorders. The main objective of the research work was to develop simple, rapid and sensitive analytical method using RP- UFLC method for estimation of synthetic steroid hormone danazol in bulk and finished formulation. Methodology: A simple, rapid and sensitive analytical method was developed. Separation of the chromatographic condition was done by using a mobile phase as isocratic flow with the concentration of 25 mM ammonium acetate (pH 4) and acetonitrile at the ratio of 50:50v/v. Flow rate of the mobile phase was maintained at l ml/min. Stationary phase used was C18 column (250 x 4.6 mm). Detection of danazol drug was carried out at 275 nm. The developed method was evaluated for the validation parameters such as specificity, sensitivity, linearity, accuracy, precision, ruggedness and robustness were performed as per the ICH guidelines. Outcome: An optimized chromatographic condition for danazol drug was achieved and results showed good peak resolution. The developed method for the danazol can be used for the quantitative and qualitative estimation of bulk and finished formulations
Authors and Affiliations
Shanmugam R, Lalitha Priyanka D, Kirthi A, Ashok Kumar C K
Keywords
Related Articles
- EP ID EP343865
- DOI 10.15254/H.J.D.Med.8.2016.154
- Views 55
- Downloads 0
NEWER ANTIPLATELET AGENTS IN CARDIOVASCULAR DISEASE
Plan: Antiplatelet drugs are the corner stone in the treatment of cardiovascular disease. They remain the main stay in preventing aberrant platelet activation in pathophysiological conditions like myocardial infarction,...
A COMPARATIVE EVALUATION OF IN VITRO ANTACID ACTIVITY OF TWO Tephrosia SPECIES USING MODIFIED ARTIFICIAL STOMACH MODEL.
Plan: The methanol extracts of Tephrosia calophylla and Tephrosia maxima (Fabaceae) roots were assessed for the antacid activity by using self-fabricated modified artificial stomach model. Preface: Tephrosia calophylla a...
TRADITIONAL AYURVEDIC TABLETS MAY CONTAIN HEAVY METALS
Plan: Several authors have reported the presence of alarmingly high levels of heavy metals like lead, arsenic, cadmium and mercury in ayurvedic medicines. Ayurveda employs several classes of medicines, among which gulik...
DOSAGE ADJUSTMENTS OF POTENTIALLY HEPATOTOXIC MEDICATIONS IN PATIENTS WITH LIVER DYSFUNCTION
Plan: To assess the incidence of inappropriate dosing of drugs having side effects like hepatotoxicity in hospitalized patients with hepatic impairment. Preface: Inappropriate dosing in patients with liver dysfunction...
PROTECTIVE EFFECT OF AN HERBAL EXTRACT IN AMLODIPINE INDUCED TESTICULAR DYSFUNCTION IN RATS
Plan: To verify the protective effects of the ethanolic extract of Asteracantha longifolia seeds in Amlodipine induced testicular dysfunction in rats. Preface: The widely used Dihydropyridine type calcium channel blocker...