DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR FLUCONAZOLE AND IVERMECTIN IN TABLET FORMULATION BY USING RP-HPLC
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 8
Abstract
A simple, efficient and reproducible RP-HPLC method for the simultaneous determination of Fluconazole and Ivermectin in bulk and tablet formulation has been developed and validated. The separation was carried out using Insertil C18 column (250 mm x 4.6 mm, 5 µ) using Acetonitrile:Methanol:Water (75:15:10 v/v/v) as mobile phase. The flow rate was 1.5 ml/min and detection was carried out 254 nm. The retention time of Fluconazole and Ivermectin were 2.313 and 9.617 respectively. The linear was established in the range of 50-150 µg/ml and 52-156 µg/ml for Fluconazole and Ivermectin respectively. Percentage recoveries for Fluconazole and Ivermectin were found to be 99.830±1.079 and 100.814±1.99 respectively. All the analytical validation parameters were determined and found in the limit as per ICH Guidelines which indicate the validity of the method. The developed method is also found to be precise and robust for the simultaneous determination of Fluconazole and Ivermectin in tablet formulation.
Authors and Affiliations
Sanjay Harod , Nimita Manocha , Ashwin Hingole , P. K. Dubey
FORMULATION AND IN-VITRO EVALUATION OF NOVEL BUCCAL MUCOADHESIVE TABLETS OF FELODIPINE
The objective of this work was formulation and in-vitro evaluation of novel buccal mucoadhesive tablets of felodipine as core in cup to release and permeate the drug unidirectionally towards the buccal mucosa and to ente...
A REVIEW ON ADVANCES OF SUSTAINED RELEASE DRUG DELIVERY SYSTEM
Sustained release matrix tablets facilitate prolonged and continuous drug release and improve the bioavailability of drugs while avoiding unwanted side effects. Ofloxacin is a broad spectrum antibacterial agent used for...
MEDICINAL USES AND PHYTOCONSTITUENTS OF PAEONIA OFFICINALIS
Paeonia officinalis (European peony, Common peony) has been cultivated in Europe for years. The root has been used medicinally for over 2,000 years mainly in the treatment for epilepsy and to promote menstruation. Root i...
ANTIBACTERIAL POTENTIAL OF CRUDE METHANOLIC EXTRACT OF Leonotis nepetifolia (L) R. Br
The methanolic extract of leaf, flower and stem of Leonotis nepetifolia was analyzed for antibacterial activity against eight pathogenic bacterial isolates viz., Escherichia coli, Pseudomonas aeruginosa, Shigella dysente...
DRONEDARONE: A NEW MOLECULE FOR ATRIAL FIBRILLATION
Atrial fibrillation is one of the most common serious cardiac rhythm disturbances and is responsible for substantial morbidity and mortality. Efficacy and safety of currently employed antiarrhythmic drugs continue to be...