DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR ESTIMATION OF MOZAVAPTAN IN HUMAN PLASMA USING LC-MS/MS
Journal Title: International Journal of Pharmaceutical and Biological Research - Year 2011, Vol 2, Issue 2
Abstract
A simple, accurate liquid chromatography with tandem mass spectrometry (LC/MS-MS) method has been developed and validated in human plasma. The method employed liquid-liquid extraction. Samples containing Mozavaptan were chromatographed on a Intersil ODS-2 column (5μm, 150 x 4.6 mm) at a temperature of 40°C. The isocratic mobile phase composition was a mixture of Methanol / 0.1% formic acid), which was pumped at a flow rate of 1.0 mL / min with split ratio of 90:10.The retention time under these chromatographic conditions was found to be 1.0 minute with run time 2.6 minute. Diethyl ether was found to be good extracting and produced a satisfactory chromatogram. The developed LC/MS-MS method was found to be selective, simple, sensitive, accurate and linear for the analysis of Mozavaptan in human plasma. The retention time and inturn run time was very short, hence required less mobile phase for the method, making it more economical and rapid. The method was applicable for the pharmacokinetic study of Mozavaptan
Authors and Affiliations
PATEL ANKUR P. * , AKHTAR JAWED
Keywords
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