SPECTROPHOTOMETRIC ESTIMATION OF PROPAFENONE IN BULK DRUG AND ITS PHARMACEUTICAL FORMULATION
Journal Title: International Journal of Pharmaceutical and Biological Research - Year 2010, Vol 1, Issue 2
Abstract
Three simple, accurate, rapid and sensitive methods developed for the determination of propafenone in bulk drug and in its tablets by UV Spectroscopy (Method - A), Extractive Spectrophotometric estimation (Method -B) and Hydrotropic method (Method - C). In Method A propafenone estimated at 301 nm using ethanol as a solvent. The linearity was observed in the concentration range of 20 - 200 μg/ml with correlation co efficient of 0.9819. In Method B extractive spectrophotometric estimation of propafenone using methylene blue and phosphate buffer (PH – 8) as an ion pair complexing agent using chloroform as a extractive solvent, the intensity of the resulting bluish green color of the organic layer was measured at 485 nm. The linearity was observed in the concentration range of 200 - 1000 μg/ml with correlation co efficient of 0.9769. In Method C Diphen hydramine citrate solution used as a hydrotropic agent to dissolve the propafenone which is water insoluble, and it is estimated at 301 nm with the linearity range and correlation co -efficient of 15 – 150 μg/ml and 0.9985. The result of analysis for all the methods was validated statistically and by recovery studies.
Authors and Affiliations
B. DHANDAPANI , J. KEERTHI , D. L. PRIYAMVADHA , BH. ANUSHA , M. BABU NAIK , K. K MANJEERA , R. V. CELESTIN BABOO , L. LAVANYA
Keywords
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