DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR ESTIMATION OF RIVAROXABAN USING HPLC-PDA IN HUMAN BLOOD PLASMA
Journal Title: Journal of Drug Delivery and Therapeutics - Year 2017, Vol 7, Issue 7
Abstract
Rivaroxaban is oral anti-coagulant drug, used in the treatment of various blood disorders. A sensitive and accurate liquid chromatographic methodfor the quantification of rivaroxaban in human plasma was successfully developed. Protein precipitation was used for sample preparation. Quantification of analyte was achieved on phenomenexluna C8 (5µm x 25cm x 4.6mm i.d.) column using methanol:water:dimethyl sulfoxide (50:45:5, v/v/v) as mobile phase at a flow rate of 1ml/min. Detection was achieved at 252nm over the linearity concentration range of 5-40µg/ml. Retention time was found 6.2 min. The LOD and LOQ were found to be 1.5 µg/ml and 4.5 µg/ml, respectively.The developed method was validate as per US FDA guidelines.
Authors and Affiliations
Satyendra Yadav
Keywords
Related Articles
- EP ID EP300612
- DOI -
- Views 72
- Downloads 0
Management of Grahani Roga by Ayurveda principles and life style modification
Grahani is an ayurveda terms related to the seat of agni (digestive fire), which help in the metabolism and digestion of food. The ancient text of ayurveda described that ingestion, digestion, absorption and assimilation...
Formulation and evaluation of chitosan containing mucoadhesive buccal patches of metoprolol succinate
Mucoadhesive buccal patches containing metoprolol succinate were prepared using the solvent casting method. Chitosan was used as bioadhesive polymer and different ratios of chitosan to PVP K-30 were used. The patches wer...
NOVEL FORMULATION STRATEGIES FOR INSULIN DELIVERY
Insulin is a hormone made by the pancreas that allows your body to use sugar (glucose) from carbohydrates in the food that you eat for energy or to store glucose for future use. Successful oral insulin delivery involves...
SOLUBLITY ENHANCEMENT OF MICONAZOLE BY FORMULATION OF HYDROTROPIC SOLID DISPERSIONS
In the present research work mixed hydrotropic solubilization phenomenon is used to enhance the solubilization of poorly water-soluble drugs. Bulk drug samples were identified by the observed IR spectra and the melting p...
ARE THE SIDE EFFECTS OF PARACETAMOL BOOSTED BY CAFFEINE??
Paracetamol (acetaminophen) with caffeine is a combination medicine indicated for the temporary relief of pain and discomfort associated with a number of conditions such as headache or muscle pain. Paracetamol has a narr...