Development and Validation of RP-HPLC method for determination of Modafinil in bulk and dosage form
Journal Title: International Current Pharmaceutical Journal - Year 2012, Vol 1, Issue 4
Abstract
A reverse phase high performance liquid chromatographic method was developed for the determination of Modafinil in bulk and dosage form. The separation was effective on a Hypersil ODS C18 column (250 mm x 4.6 mm; 5µ) using a mobile phase mixture of Buffer:Acetonitrile in a ratio of 55:45 (v/v) at a flow rate of 1.0ml/min. The detection was made at 220nm. The retention time of modafinil was found to be 4.80±0.06 min. Calibration curve was linear over the concentration range of 20-120µg/ml of modafinil. The proposed method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid and thus found to be suitable for the quantitative analysis of modafinil in the bulk and dosage form.
Authors and Affiliations
Chusena Bhimanadhuni, Devala Garikapati, Swetha Karamsetty
Keywords
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- EP ID EP135056
- DOI http://dx.doi.org/10.3329/icpj.v1i4.10062
- Views 122
- Downloads 0
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