Development and Validation of RP-HPLC method for Simultaneous Estimation of Metformin and Pioglitazone
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2017, Vol 5, Issue 1
Abstract
A new simple, accurate ,precise and reproducible RP-HPLC method has been developed for simultaneous estimation of metformin and pioglitazone in bulk drug form using proper HPLC system. The mobile phase consists of phosphate buffer and acetonitrile in the ratio of 35:65 at pH 3.4.The detection wavelength was carried out at 228 nm. The method was linear over the concentration range for metformin 50-100 μg/mL and for pioglitazone 20-180 μg/mL. The recoveries of metformin and pioglitazone were found to be 100.5 and 98.7% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method method was successfully for the analysis of pharmaceutical formulations containing dosage form.
Authors and Affiliations
Uttam kumar agrawal| Department of Pharmacy, Sun Rise University, Alwar, Rajasthan, India, Jeyabalan Govindasamy| Department of Pharmacy, Sun Rise University, Alwar, Rajasthan, India
Keywords
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