DEVELOPMENT AND VALIDATION OF SIMPLE UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF APIXABAN IN API AND ITS BULK DOSAGE FORM
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2017, Vol 7, Issue
Abstract
Abstract Simple, rapid, sensitive, precise and specific UV spectrophotometric method for the determination of Apixaban in bulk drug and pharmaceutical dosage form were developed and validated. In this method solution of Apixaban was prepared in methanol. Apixaban standard solution was scanned in the UV rang (400-200nm) in a 1cm quartz cell in a double beam UV spectrophotometer. The standard solution of Apixaban showed maximum absorption at wavelength 280 nm. The method obeys Beer’s law in the concentration range from 2 μg/ml -10μg/ml. The correlation coefficient was found to be 0.9967 and regression of the curve was found y = 0.0995x + 0.0149 with excellent recovery 99-104%. Limit of detection and limit of quantitation were found to be 0.186 μg/ml and 0.566μg/ml respectively. The method was validated for several parameters like accuracy, precision as per ICH guidelines. Statistical analysis proved that the methods are repeatable and specific for the estimation of the said drug. These methods can be adopted in routine assay analysis of Apixaban bulk or tablet dosage form.
Authors and Affiliations
P. A. Malode*, S. S. Pendhari, B. S. Ghuge, S. P. Anantwar
Keywords
Related Articles
- EP ID EP330753
- DOI -
- Views 129
- Downloads 0
DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DACLATASVIR AND SOFOSBUVIR IN DRUG PRODUCT BY RP-HPLC
New Analytical method was developed for the estimation of Daclatasvir and Sofosbuvir in drug product by liquid chromatography. The chromatographic separation was achieved on C18 column (Phenyl XDB 250*4.6mm) at ambient t...
A REVIEW ON DIETETICS IN AYURVEDA
Food is the essential component for living a healthy life. This is placed in Triopastambhas (Nidra, Ahara, and Brahmacharya) in ancient classics because of its great importance. The importance of food is not only confine...
ASSESSMENT OF PMTCT SERVICE QUALITY AT BEDDELLE AND METTU KARL HOSPITALS, IN ILU ABABORA ZONE OROMIYA REGION, SOUTH WEST ETHIOPIA.2015
Abstract Background: - Globally, there are an estimated 34 million people living with HIV (PLHIV), the vast majority of who live in sub-Saharan Africa and every day over 5700 persons die from AIDS. Mother-to-Child Transm...
Formulation and Evaluation of Gastroretentive Floating Microballoons of Norfloxacin
The present study involves preparation and evaluation of floating microballoons with norfloxacin s model drug for prolongation of gastric residence time. The microballoons were prepared by the solvent evaporation method...
A PHYTOPHARMACOLOGICAL REVIEW ON ARID REGION MEDICINAL PLANT- AERVA PSEUDOTOMENTOSA
Abstract Aerva pseudotomentosa Blatt. & Hallb. (Amaranthaceae) is arid region plant of Amaranthaceae family. It is commonly known as Bui. It is used in folk medicine for the treatment of various ailments such as pain and...