DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PHYTOMENADIONE IN INJECTION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 3
Abstract
The present manuscript describes simple, sensitive, accurate, precise and economical spectrophotometric method for the determination of phytomenadione in injection formulation. Method is based on the reaction of phytomenadione with 2, 4 dinitrophenyl hydrazine which produce deep yellow color. The yellow color chromogen shows wavelength of maximum absorbance at 350 nm. Linearity was obtained in the concentration range of 0.1 – 1.0 µg/ml. The method was successfully applied to pharmaceutical injection formulation because no interferences from formulation excipients were found. The suitability of this method for the quantitative determination of phytomenadione was proved by validation. The proposed method was found to be fast and cost effective and can be used for the routine quality control analysis of pharmaceutical formulations. The results of analysis have been validated statistically and by recovery studies.
Authors and Affiliations
Satish Patel , Premal Parikh
Keywords
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