Development, Characterization and Ex vivo Evaluation of Various Liposome-encapsulated Aceclofenac Formulations
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 9, Issue 4
Abstract
Aim: Formulation of aceclofenac (ACE) into liposomal gel, improving its skin permeation and potentiate its local anti-inflammatory effect. Methodology: ACE liposomes were fabricated by lipid film hydration technique. The prepared formulations were traditional liposomes containing cholesterol (F1), ultra-deformable liposomes containing Tween 60 (F2) and modified liposomes containing both cholesterol and Tween 60 (F3). All formulations were incorporated in 1% carbopol 974 as gelling agent. The developed liposomal formulations were evaluated for its particle size, zeta potential, drug entrapment efficiency percent and stability for 90 days at 4°C. While the developed liposomal gel formulations were characterized for its in vitro ACE release and ex vivo permeation through rat skin. Anti-inflammatory effect of ACE gel formulations were evaluated in rats using carrageenan induced paw edema. Results: The prepared liposomes had a mean vesicle size of 577 nm, 218 nm and 332 nm for F1, F2 and F3 respectively and entrapment efficiency percent of 76.8%, 58.3% and 66.2% for F1, F2 and F3 respectively. The stability study revealed that F1 vesicles have the highest physical stability followed by F3, while F2 showed the lowest stability in terms of vesicles size and entrapment efficiency percent. The in vitro and ex vivo results showed higher drug release and permeability from F2 and F3 than that of F1. In addition, ACE liposomal formulations have significant higher anti-inflammatory effect than that of marketed product Bristaflam cream®. Conclusion: All liposomal gel formulations have the ability to enhance ACE anti-inflammatory effect in rats paw edema in comparison with marketed product Bristaflam cream®.
Authors and Affiliations
Fathy Ibrahim Abd-Allah
Keywords
Related Articles
- EP ID EP342154
- DOI 10.9734/BJPR/2016/22501
- Views 110
- Downloads 0
Prophylactic Effect of Clofibrate on Hyperbilirubinemia in Very Low Birth Weight Twins
Aims: To determine the prophylactic effects of clofibrate on hyperbilirubinemia in very low birth weight twins. Study Design: A randomized double blind clinical trial Place and Duration of Study: Department of Neonatal...
Formulation and Evaluation of Dexibuprofen Transdermal Films in Rabbits
Dexibuprofen is a practically water-insoluble nonsterodial anti-inflammatory drug. Following previous experience on enhancing the solubility of the drug, a mixed hydrotropic solubilization technique was here applied in o...
Assessment of Mutagenic Effects of Sulfadoxine-Pyrimethamine (SP) on Animal Model
Introduction: The use of medicines in pregnancy has always been a source of concern because of adverse effects on the foetus. Aim: The study therefore investigated the mutagenic potential of sulfadoxine-pyrimethamine (SP...
Densitometric and Spectrometric Determinations of Florfenicol and Buparvaquone
Three new accurate and sensitive methods were developed for the determination of two veterinary drugs. Florfenicol was analyzed by a stability-indicating TLC/densitometric method in presence of its amine degradation prod...
Antimicrobial Activity of Ethanolic Crude Extracts of Thespesia populnea Flowers
Aim: To analyze the antimicrobial activity of ethanolic crude extract of Thespesia populnea Flowers. Study Design: Antimicrobial activity of Thespesia populnea was carried out using disc diffusion method against crude ex...