Development of a New Rapid, Efficient and Reproducible Reverse Phase - HPLC Method for the Analysis of Decitabine in Bulk and Tablet Dosage Form
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2017, Vol 5, Issue 4
Abstract
Development of a new rapid, efficient and reproducible reverse phase-HPLC method for the analysis of Decitabine in bulk drug and tablet dosage form. This separation was achieved by using Develosil ODS HG-5 RP column 150mm x 4.6mm, 5μm (particle size) i.d. in isocratic mode, with mobile phase containing ACN : Acetate buffer (55:45), adjusted to pH 3.9 using ortho phosphoric acid. The flow rate was maintained at 1.0 ml/min and analyte were monitored at 254 nm. The retention time of Decitabine was found to be 2.97 min. The linearity for Decitabine were in the range of 0-60 μg/ml. The recoveries of Decitabine were found in the range of 100.4933%, 101.4733% and 100.0467%. The developed method was validated as per ICH guidelines and successfully applied to the further analysis of Decitabine in bulk and tablet dosage form
Protective Role of Beta vulgaris Peel Extract in T4- Induced Hyperthyroidism in Male Mice
Objective:The hitherto unknown effects of peel extract of Beta vulgaris root in regulating hyperthyroidism was investigated in male albino mice. Method: Hyperthyroidism was induced by L-thyroxine(L-T4 ) at a pre-standard...
Antidiabetic and Antioxidant Properties of Waltheria indica L., an Ethnomedicinal Plant
The present investigation was carried out to study the antidiabetic activity of methanolic extract of entire plant of Waltheria indica L. and its effect on the lipid profile, antioxidant properties and histopathology of...
To Compare Cost and Effectiveness of Emperically Treated Antibiotic Magnex Forte and Cepez-SB in the Treatment of Acute Gastroenteritis
Background: Health systems have multiple goals, but the fundamental reason they exist is to improve health. Cost And Effectiveness Analysis (CEA) is one tool decision-makers can use to asses and potentially improve the p...
Effect of delay of fixation on mitotic counts in histopathological sections of tumors
Mitotic count is the most commonly used method of assessing the proliferative activity of a tumor. Mitotic count should be done very meticulously because it is used to evaluate prognosis of various tumors and is one of t...
Effect of Ficus benghalensis Linn. Root Extracts on Freund’s Adjuvant Induced Arthritis in Rats
Aim: The present study was carried out to evaluate the anti-arthritic activity of Ficus benghalensis root extracts on Freund’s adjuvant induced arthritis in rats. Method: The crude root extracts was administered orally a...