Diagnostic assessment of liver enzymes in blood profiles from dairy cows
Journal Title: Revista MVZ Cordoba - Year 2013, Vol 18, Issue 2
Abstract
Objective. The aim of this study was to establish the association, sensibility (Se) and specificity (Sp) of the plasmatic activity of glutamate dehydrogenase (GDH, EC: 1.4.1.3), aspartate aminotransferase (AST, EC: 2.6.1.1) and g-glutamil transpeptidase (GGT, EC: 2.3.2.2) as liver damage markers in blood profiles of dairy cows. Materials and methods. The records of the plasma activity for GDH, AST and GGT obtained from blood profiles taken from 1566 cows from 112 dairy herds. The association between enzymatic activity, area below the curve (ABC), and Se and Sp using a ROC curve were determined, considering liver damage when GDH >30 U/L or GGT >39 U/L. Results. The AST was more likely associated to GDH (r=0.55) than to GGT (r=0.44). AST also had a higher ABC (p=0.01) when using GDH >30 U/L as an indicator of liver damage. The correlation between AST and GGT was of r=0.42. AST Se to detect liver damage with a cut-off level of >150 U/L was lower than the Se of GGT. The critical point for maximum Se and Sp of the ROC curve was obtained with AST>110 U/L and an increase in the diagnostic concordance with GDH (Kappa>0.300), was obtained using this value. Conclusions. Plasma activity of AST is more associated to GDH than to GGT in dairy cows. The cut-off level of plasma activity of AST for liver damage prediction in dairy cows would be >110 U/L.
Authors and Affiliations
Mirela Noro, Ph. D *| Universidade Federal do Pampa, UNIPAMPA, Curso Medicina Veterinaria. Uruguaiana, Brasil.Correspondencia: mirelanoro@gmail.com, Pía Cid T, Esp| Universidad Austral de Chile, UACh, Instituto Ciencias Clínicas Veterinarias. Casilla 567, Valdivia, Chile., Catalina Wagemann F, M. Sc| Universidad Austral de Chile, UACh, Instituto Ciencias Clínicas Veterinarias. Casilla 567, Valdivia, Chile., Verónica Arnés V, Tec Med| Universidad Austral de Chile, UACh, Instituto Ciencias Clínicas Veterinarias. Casilla 567, Valdivia, Chile., Fernando Wittwer M, M. Sc| Universidad Austral de Chile, UACh, Instituto Ciencias Clínicas Veterinarias. Casilla 567, Valdivia, Chile.
Keywords
Related Articles
- EP ID EP3308
- DOI -
- Views 370
- Downloads 16
AGRONOMIC EVALUATION OF FORAGES SORGHUM (Sorghum vulgare) AND MILLO GENOTYPES FOR FEEDING BOVINE IN THE SINÚ VALLEY
Objective. To evaluate seven millo and five sorghum genotypes to determine forage yield and to use it in feeding bovine in Sinú valley, Colombia. Materials and methods. Sorghum (Sorghum vulgare) and millo seeds were hybr...
Chemical and organoleptic evaluation of maralfalfa silage (Pennisetum sp.) plus fresh cassava (Manihot esculenta)
Objective. To evaluate the chemical composition and the organoleptic traits of maralfalfa silage (Pennisetum sp.), containing different proportions of fresh cassava (Manihot esculenta). Materials and methods. Four treatm...
STRUCTURAL ANALYSES OF CUT CACHAMA HYBRID FILLETS Piaractus branchypomus x Colossoma macropomum USING BACTERIOCINS PRODUCED BY Lactobacillus plantarum LPBM10 VACUUM PACKED
Objective. To determine microstructure, texture and sensory changes in cut cachama hybrid fillets. Materials and methods. Hybrid fillets that were vacuum packed for 30 days at 3°C were analyzed after preservative treatme...
Oxidized lipids intake: effect on antioxidative enzimatic activity in rainbow trout Oncorhynchus mykiss (Walbaum)
Objective. To determine the effect, of different oxidation level of lipid, ingested for a period of 20 and 90 days on Catalase (CAT), Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) activities in hepatical an...
Use of ground and extruded canola seeds in feed for 15-30 kg piglets
Objective. Determine the nutritional values of ground (SCI) or extruded (SCE) canola seed and evaluate its use in the performance of piglets from 15 to 30 kg. Materials and methods. Two experimental diets with canola see...