Diagnostic Value Of Resistin And Hepcidin In Neonatal Sepsis
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 3
Abstract
Diagnosis of sepsis-attributed morbidity and mortality in the neonates still constitute a great challenge. Several attempts have been described to evaluate new diagnostic markers. Resistin and hepcidin are relatively new biomarkers that have been investigated and suggested as possible markers of infection in adult. The aim of this study was to evaluate hepcidin and resistin as new biomarkers of neonatal sepsis (NS) in comparison to the established diagnostic methods as C-reactive protein (CRP), blood culture and detection of bacterial 16S rDNA by PCR. The study included 150 neonates with suspected NS who were subjected to sepsis screen scoring. Complete blood count, CRP and automated blood culture were performed. Broad range bacterial 16S rDNA was detected by PCR without prior enrichment. Resistin and hepcidin serum levels were measured by enzyme immunoassay. Klebsiella was the most common isolated organism (9 isolates) followed by coagulase negative Staphylococci (8 isolates). PCR results for 16S rDNA were positive in 40 cases. Resistin level was significantly elevated in 84 cases; 80 of the sepsis groups and 4 in the control group. Hepcidin level was found to be elevated in 62 cases; 59 of the sepsis groups and 3 cases of the control group. Both resistin and hepcidin levels were significantly elevated in neonates with sepsis and were positively correlated with CRP and band cells count. However, resistin had greater diagnostic value compared to hepcidin. Both of these markers had an efficacy comparable to that of CRP in the diagnosis of NS.
Authors and Affiliations
Khalifa AS, Mabrouk MM, Al-Zahrani Akh, Taha AA, Eed EM
Keywords
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- DOI 10.9790/264X-0403011621.
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