Differential effects of genistein, estradiol and raloxifene on rat osteoclastsin vitro.
Journal Title: Pharmacological Reports - Year 2005, Vol 57, Issue 3
Abstract
Genistein, a major phytoestrogen of soy, is considered a potential drugfor prevention and treatment of postmenopausal osteoporosis. It is not clear whether mechanism of actionof genistein on bone turnover is distinct from that of estradiol or raloxifene. The aim of the presentstudy was to investigate the effects of genistein on the formation of osteoclasts from neonatal rat bonemarrow cells in vitro, and compare them with those of estradiol and raloxifene. Formation of osteoclastswas stimulated by 1,25-dihydroxyvitamin D(3), added to the culture media on the second day after plating,together with genistein (10(-8)-10(-5) M), estradiol (10(-10)-10(-7) M) or raloxifene (10(-9)-10(-6)M). The bone marrow cell culture lasted 7 or 9 days. Number of osteoclasts and number of osteoclast "ghosts"(necrotic giant cells) were determined. Genistein, estradiol and raloxifene, at some concentrations,decreased the number of osteoclasts after 9-day culture of bone marrow cells. Genistein decreased thenumber at 10(-8) M because of decreasing the viability of osteoclasts, whereas at 10(-5) M due to attenuationof osteoclast formation. Estradiol decreased the osteoclast number at 10(-9) M due to decreasing theirviability, whereas at 10(-8) and 10(-7) M it was the effect of both decreasing the viability and inhibitionof the formation. Decreases in the number of osteoclasts caused by raloxifene (10(-9), 10(-8) M werethe effect of decreasing the viability of these cells.
Authors and Affiliations
Leszek Śliwiński, Joanna Folwarczna, Waldemar Janiec, Grzegorz Grynkiewicz, Katarzyna Kuzyk
Keywords
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