Differential gene screening and prognostic analysis of key protein regulatory genes in different stages of pancreatic cancer
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 5
Abstract
Objective To analyze the differentially expressed genes and related enriched pathways in the low-stage and high-stage groups of pancreatic cancer, and to explore the role of key regulatory proteins in the development of pancreatic cancer combined with prognostic information, so as to provide theoretical support for a deeper understanding of the molecular mechanism of pancreatic cancer in the high-stage group. Methods This study used the transcriptome data of pancreatic cancer with different tumor stages in the TCGA database to screen the differentially expressed genes, and performed functional enrichment analysis and protein-protein interaction network construction. Cytoscape software was then used for visualization and module mining to obtain key protein regulatory genes. Combined with the corresponding clinical information, the survival curves of key genes were drawn. Results A total of 277 differential genes were screened, including 30 up-regulated genes and 247 down-regulated genes. Differential genes were mainly related to digestion, humoral immune response, intestinal cholesterol absorption and metabolism processes of some substances, related to blood microparticles, endoplasmic reticulum lumen and other cell components, and related to molecular functions such as serinerelated enzyme activity, sugar binding and triglyceride lipase activity. The KEGG pathway was mainly related to pancreatic secretions, protein digestion and absorption, and fat digestion and absorption. Prognostic analysis showed that the expression levels of CPA1 and AMY2B genes were significantly correlated with the overall survival time of patients with pancreatic cancer (P < 0. 05) . Conclusion The key protein regulatory genes CPA1 and AMY2B are closely related to the occurrence and prognosis of pancreatic cancer in different stages, providing new ideas for precise treatment of patients with high-stage pancreatic cancer in the future.
Authors and Affiliations
HAN Weiguang, WANG Qing
Keywords
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