Direct multiple shoots proliferation of black night shade (Solanum nigrum l.) from shoot tip explants induced by thidiazuron
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2015, Vol 3, Issue 1
Abstract
The present investigation was undertaken in Black Night Shade (Solanum nigrum L.) which is an important medicinal plant. Direct multiple shoots proliferation was achieved from shoot tip. The shoot tips were cultured on MS medium fortified with Thidiazuron (TDZ) (1.0-7.0 mg/L) for multiple shoot induction. Multiple shoots proliferation was best observed at 3.0 mg/L TDZ from the shoot tip explants within three weeks of culture. Shoot number per explant ranged between 2 and 10. Individual shoots were aseptically excised and sub cultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Acetic Acid/Indole Butyric Acid (IAA/IBA) (0.5mg/L–2.0mg/L) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 86%. Plants looked healthy with no visually detectable phenotypic variations.
Authors and Affiliations
Ugandhar. T| Department of Botany, SRR Govt. Arts & Science College Karimnagar – 505001, India, Sammaiah. D| Department of Botany, Govt Degree College Huzurabad – 505498, India, Ayodhya Ramulu. Ch| Regional Institute of Education, Unit IX, Sachiwalaya marg, Bhubaneswar- 751 022,India, Anitha Devi. U| Department of Botany, Govt Degree & P.G. College for Women Karimnagar- 505001,India, Balaraju. P| Department of Botany, Govt. Degree & PG College Peddapally – 505172, India
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