DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period
Journal Title: Open Veterinary Journal - Year 2012, Vol 2, Issue 6
Abstract
This study was designed to investigate the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in dairy cattle during the transition period. Blood samples were collected from 4 dairy cattle at 3 weeks before the expected parturition (wk -3), parturition (wk 0) and 3 weeks after parturition (wk +3). The DNA damage of PBMC and PMN was evaluated based on the comet assay using visual scoring (arbitrary units). Undamaged DNA remained within the core (score 0) and the broken DNA migrated from the core towards the anode forming the tail of a comet (scores 1-4). Significantly higher scores in PBMC at wk 0 and wk +3 were observed compared with those in PMN although there were no significant changes of scores in either cell type during the experimental period. It is suggested that the apoptotic rate of PBMC is accelerated compared with that of PMC during the transition period.
Authors and Affiliations
M. Tharwat| Department of Large Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan,Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Egypt, D. Endoh| Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University, 582 BunkyodaiMidorimachi, Ebetsu, Hokkaido 069-8501, Japan, S. Oikawa| Department of Large Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan
Keywords
Related Articles
- EP ID EP9264
- DOI -
- Views 396
- Downloads 25
Effects of Watercress (Nasturtium nasturtium) extract on selected immunological parameters of rainbow trout (Oncorhynchus mykiss)
Watercress (Nasturtium nasturtium) is a medical plant containing diverse chemically-active substances with biological properties. The present study was conducted to investigate the immunomodulatory effects of watercress...
Comparison between fish and linseed oils administered orally for the treatment of experimentally induced keratoconjunctivitis sicca in rabbits
The objective of this study was to compare the efficacy of two sources of omega 3 and 6, fish oil (FO) and linseed oil (LO), orally administered, alone or in combination, for treating experimentally induced keratoconjun...
Simultaneous pyometra and viable puppies’ gestation in a bitch
Here we describe a case of pyometra coexisting with gestation in a 4.5 year-old miniature short-haired Dachshund. The dog exhibited depression, vaginal discharge, polydipsia and dehydration. Ultrasound examination revea...
Physical and morphometric characterization of indigenous cattle of Assam
The present investigation was undertaken to study the physical and morphometric characteristics in indigenous cattle of Assam. The data pertain to 339 indigenous cattle of different categories. The physical characterist...
Antibiogram and heavy metal tolerance of bullfrog bacteria in Malaysia
Bacterial isolates from 30 farmed bullfrogs (Lithobates catesbeianus) weighing 500-600 g at Johore, Malaysia with external clinical signs of ulcer, red leg and torticollis were tested for their antibiograms and heavy me...