Effect of Chitosan in Radical Scavenging and Bactericidal Activity Isolated from Agaricus bisporus Mushroom
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 4
Abstract
In the present study, Chitin has been extracted from Agaricus bisporus (Button mushroom). The obtained chitin was converted into the more useful chitosan and the crude chitosan extract was measured for its absorption maxima by UV Spectrophotometer and the maximum peak at 265 nm was observed. FT-IR spectroscopy was done to identify the functional groups presented in the chitosan which was analyzed between the ranges of 4000–400 cm-1. Chitosan was characterized by significant amide bands at 3265.49 cm−1. The absorbance bands of 1402.25, 1153.43, 900.76 and 445.56 cm-1 indicated CH2 stretching, CH stretching, C=O stretching in secondary amide respectively which confirms the structure of chitosan. The antioxidant activity of chitosan was determined by DPPH free radical scavenging assay and the value gained was 65.90% at 250 mg/ml, which was due to the presence of rutin, gallic acid, caffeic acid and catechin in the phenolic composition of Agaricus bisporus. Finally, in vitro antibacterial screening of chitosan from Agaricus bisporus was performed against selected clinical isolates and the zone of inhibition shown highest activity in Bacillus subtilis, P. aeruginosa followed by K. pneumonia, and Acinetobacter baumannii. These findings suggested that the Agaricus bisporus act as the potential source to produce eco-friendly chitin and chitosan in the development of drugs, artificial bone and raw material for food industries in near future.
Authors and Affiliations
Gayathri Murugan Vairamuthu, Josephin Jancy Rani Peter, Avila Jerley, Srinivasan Dhandapani
Keywords
Related Articles
- EP ID EP671041
- DOI 10.21276/ijlssr.2018.4.4.14
- Views 111
- Downloads 0
Antioxidant and Hepatoprotective Activities of Carica papaya (Papaw Leaf) and Loranthus bengwensis (Cocoa Mistletoes) against Diclofenac Induced Hepatotoxicity in Rats
In this study, the effect of diclofenac sodium induced liver damage in rats was investigated, biochemically. Damaging effects of reactive oxygen species (ROS) on living systems are well documented, they include oxidative...
Analysis of Changing Vegetation Pattern Under Different Climatic, Edaphic and Altitudinal Factors of Doon Valley, Uttarakhand, India
The present study was conducted in altitudinal variable hight, and soil pH growing vegetation of Doon valley that the soil type found in Mussoorie is generally medium loamy but its composition, moisture, and pH generally...
Effect of Sex Hormones and Prolactin on Sickle Cell Erythrocyte Polymerisation in Portharcourt, Rivers State, Nigeria
Sickle cell disease (SCD) is an inherited hematological disorder that causes red blood cells to break down continuously. This leads to a rigid, sickle like shape under certain conditions, causing polymerization of the si...
Antimicrobial activity of Antibiotics and Antiseptics (Dettol and Betadine) against Clinical Isolates of Pseudomonas aeruginosa
Hundred samples viz. urine, blood, wound, pus and sputum collected from different patients were found to harbour Pseudomonas aeruginosa (P. aeruginosa) (27%) with a maximum isolation from wound samples (33.33%) and minim...
Expression and Purification of Nisin in Escherichia coli
Fusion expression is a promising strategy for the production of bioactive peptides in Escherichia coli to enhance either soluble protein level or purification potential. Nisin is the bacteriocin that had been extensively...