Effect of cholesterol exposure on the expression of metallothionein in human retinal pigment epithelial cells
Journal Title: Eye Science (Yanke Xuebao) - Year 2021, Vol 36, Issue 2
Abstract
[Objective:] To study the effects of cholesterol, the main component of drusen, on the expression of metallothionein of ARPE-19 cells. [Methods:] The ARPE-19 cell line was cultured in vitro, and the cells were divided into a control group and a cholesterol treatment group (2.5 mg/mL). The treatment time was 0, 6, 12, 24, 48, 72 hours. Real-time quantitative PCR was used to detect the expression of hMT1a, hMT2a and hMT3 at the mRNA level, and Western blot was used to detect the expression at the protein level. [Results:] The mRNA expression of hMT1a, hMT2a and hMT3 were up-regulated by cholesterol and the protein expression of total MTs was increased with cholesterol treatment. [Conclusion:] Cholesterol, the main component of drusen, can up-regulate the expression of metallothionein in human retinal pigment epithelial cells, suggesting that the expression of metallothionine can be stimulated by the initial stage of drusen formation. However, whether its detection can be used for the early detection and early diagnosis of age-related macular degeneration or not still needs to be further explored.
Authors and Affiliations
Min ZHANG, Peng ZHANG, Hongli ZHAO
Keywords
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- EP ID EP763475
- DOI 10.3978/j.issn.1000-4432.2020.12.07
- Views 14
- Downloads 0
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