Effect of Desiccation and Chilling Treatment on Somatic Embryo Development and Germination in Rough Lemon (Citrus jambhiri Lush)
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 2
Abstract
The goal of this study was to evaluate the effect of desiccation and chilling treatments on somatic embryogenesis of rough lemon (Citrus jambhiri Lush.). Styles were cultured on seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and Malt Extract for cell proliferation and somatic embryo development. Cell proliferation was maximum on MS IV media but maximum cultures showing somatic embryogenesis (52.08 %) was observed on MS VII media. Embryogenic callus proliferated on MS VII media was subjected to desiccation and chilling treatment for 24, 48, 72 and 96 hours. Embryogenic callus desiccated for 24 and 48 hours in sterile petriplates showed 58.33 and 56.94 % somatic embryogenesis respectively as compared to undesiccated callus (51.98%). Average number of cotyledonary embryos (6.80/culture) in embryogenic cultures from desiccated callus (48 hrs) was more as compared to untreated callus (2.26/culture). There was significantly less number of abnormal embryos (0.60-0.53/culture) in desiccated callus for 48, 72 and 96 hours as compared to untreated callus (7.20/culture). Chilling treatment also improves the average number of cotyledonary embryos and reduces the abnormal development of embryos. Among all the treatments desiccation of embryogenic callus for 48 hrs proved beneficial for improvement of somatic embryo development and germination.
Authors and Affiliations
Balwinder Singh
Keywords
Related Articles
- EP ID EP237193
- DOI 10.9734/BBJ/2014/6241
- Views 155
- Downloads 0
Hepatic Gene Expression of Insulin like Growth Factor and Selected Antioxidants in Diabetic Rats Treated with Turmeric or Black Cumin Seed
The effects of black cumin seed or curcumin on blood glucose level, lipid peroxidation and gene expression of selected antioxidant enzymes and insulin like growth factor-1 (IGF-1) in diabetic rats were studied. Thirty tw...
Molecular Characterization and Potential of Fungal Species Associated with Cassava Waste
Aims: Efficient utilization of cassava waste for value addition depends largely on proper understanding of its true microbial diversity. The aim of this study was to characterize using molecular methods, fungal species a...
Analysis of Gene Expression Associated with Copper Toxicity in White Birch (Betula papyrifera) Populations from a Mining Region
The Greater Sudbury Region (GSR) is one of the most ecologically disturbed regions of Canada. Recent studies have shown that Betula papyrifera accumulate metals in roots or leaves. The main objectives of the present stud...
Biddulphia (Odontella) sp. Isolated from the Seawater of Kayalpattinam Coastal Area, Gulf of Mannar and Maintained in Tuticorin Research Centre of ICAR-CMFRI
Aim: Present study was aimed to isolate marine algae from the sea water samples of Kayalpattinam Coastal area, Gulf of Mannar. Methodology: Seawater samples were collected for the isolation of phytoplankton from the Kay...
Mass Spectrometric Determination of the Primary Structure of β Amylase Isolated from Amylolytic Nigerian Maize Cultivar
This study was carried out after a five day germination period on TZEE*TZEE-W*DEMARSCUS*TZEE-W one of the most recommended high amylolytic Nigerian maize cultivar. Purification steps comprising of fractional precipitatio...