Effect of Different Extraction Techniques of Persicaria odorata Extracts Utilizing Anti-bacterial Bioassay
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 18
Abstract
Persicaria odorata is a common plant and well known in Malaysia as “Daun kesum” that is commonly used in cuisines and has various medicinal properties. This study was conducted to investigate the antimicrobial activity and the extraction technique that produce the most active plant extract. The leaves were extracted using decoction, maceration assisted by ultra-sonication and percolation with soxhlet extractor to produce the respective extracts. All extracts were tested against four bacterial strains which included gram positive and gram negative bacteria using disc diffusion method. In this research gentamicin 10 μg were used as the antibacterial standard. The antimicrobial activity of the active extract was evaluated quantitatively using three different concentrations. The result from this study shows that Persicaria odorata leaves have high potential to be used as natural antibacterial agent against some bacterial infections depending on the method used to extract the active ingredient. The results shows that the extract obtained with percolation with soxhlet technique shows the best antibacterial activity followed by maceration with ultrasonication. Decoction extracts shows the weakest antibacterial activity. The extract obtain from both maceration with ultra-sonication and percolation using soxhlet extractor show significant (P=0.05) antimicrobial activity against all four bacteria (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Salmonella spp.).
Authors and Affiliations
Rasha Saad, Jiyauddin Khan, Vivegananth Krishnanmurthi, Fadli Asmani, Eddy Yusuf
Keywords
Related Articles
- EP ID EP344692
- DOI 10.9734/BJPR/2014/12232
- Views 140
- Downloads 0
Effect of Aqueous Extract of Guava (Psidium guajava) Leaf on Blood Glucose and Liver Enzymes in Alloxan Induced Diabetic Rats
Aim: The aim of this study is to determine the effect of aqueous extract of Psidium guajava leaf on blood glucose level and liver enzymes of alloxan –induced diabetic rat. Study Design: The animals were grouped into six...
Dynamic Changes in Antioxidant Activity during Floral Development of Couroupita guianensis
Aims: To establish and optimize the in vitro antioxidant activity of methanolic extract of flower of C. guianensis at different developmental stages. Place and Duration of Study: Plant Physiology and Pharmacognosy Resea...
In vivo Anti-trypanosomal Evaluation and Phytochemical Analysis of Methanol Extract of Nauclea latifolia
Aim: Trypanosomiasis is a pathological condition that requires serious attention. The dried root of Nauclea latifolia was investigated to determine the anti-trypanosomal activity on groups of Wistar rats. Methods: A 100...
Phytochemical and Antimicrobial Properties of the Hexane and Ethanol Leaf Extract of Pterocarpus Erinaceous poir (Fabaceae)
This study evaluated the phytochemical and antimicrobial properties of the hexane and ethanol leaf extract of Pterocarpus erinaceous poir (Fabaceae). Ethno medicinally, the leaves are used in treatment of conjunctivitis,...
A New Antigen Delivery Vehicle Candidate: Orthochirus iranus Scorpion Venom Entrapped in Chitosan Nanoparticles
Aims: Antivenom has been used successfully for more than a century as the only effective treatment for scorpion stings. Orthochirus iranus scorpion venom has high toxicity and preparation of antivenom is necessary for th...