Dynamic Changes in Antioxidant Activity during Floral Development of Couroupita guianensis
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 6
Abstract
Aims: To establish and optimize the in vitro antioxidant activity of methanolic extract of flower of C. guianensis at different developmental stages. Place and Duration of Study: Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri, between December 2010 and November 2011. Methodology: The antioxidant activities of methanolic extracts of C. guianensis flower of different developmental stages were investigated spectrophotometrically against DPPH, ABTS, H2O2, NO, superoxide, hydroxyl radical and lipid peroxidation, along with ferric reducing power, metal chelating and β-carotene bleaching assay were performed. Total phenols, flavonoids and ortho-dihydric phenols were also determined by folin-ciocalteu, aluminium chloride and Arnow's reagent based standard methods respectively. Results: The methanolic extract of floral stage-I was found to possess higher level of phenolic content, flavonoids as well as ortho-dihydric phenol content. The younger floral stages, stage-I and stage-II were observed with highly potential free radical scavenging activity, while stage-III showed highest degradation rate for β-carotene bleaching assay and stage-IV showed highest anti-lipid per oxidation activity against thiobarbituric acid. Conclusion: The results demonstrated that the early floral stage showed the highest polyphenolic content and best antioxidant activity, therefore the early stages of this flower is suggested as the best harvest stage for medicinal purposes. Further studies are required for the isolation of active principles responsible for its antioxidant activity.
Authors and Affiliations
Saran Kumar Gupta, Mitali Ghosal, Dibakar Choudhury, Palash Mandal
Keywords
Related Articles
- EP ID EP344832
- DOI 10.9734/BJPR/2014/8106
- Views 84
- Downloads 0
Slow-Stroke Back Massage Intervention for Relieving Postpartum Fatigue in Primiparous Mothers after a Natural Delivery: A Randomized Clinical Trial in Zabol
Introduction: Fatigue is one of the most prevalent phenomenon reported by post-partum mothers and have various effects on their physical, emotional and cognitive aspects. Back massage relaxation techniques...
Evaluation of the Haemostatic Activities of Sida corymbosa in Rats
Aims: To evaluate the anti-haemorrhagic activity of the leaf extract of Sida corymbosa in Wistar albino rats, a plant used to arrest bleeding in ethnomedical practices. Methods: The acute toxicity test was carried out i...
Antioxidant Activity, Total Phenolic and Flavonoid Contents of Essential Oils of Three Cyperus Species (Cyperaceae)
Aims: To determine the in vitro antioxidant activity, total phenolic and flavonoid contents of essential oils from the rhizomes of Cyperus distans, Cyperus papyrus and Cyperus rotundus collected from different locations....
Influence of Process Parameters on Compressibility, Solubility and Release Characteristics of Melt Sonocrystallized Fenofibrate
Aims: The aim of the present study was to investigate the suitability of the melt sonocrystallization (MSC) technique to modify the processability properties along with solubility and drug release of anti-hyperlipidemic...
Synthesis of Silver Nanoparticles by Sodium Borohydride Reduction Method: Optimization of Conditions for High Anti-staphylococcal Activity
Aim: The aim of this research was to optimize the reaction conditions for the production of silver nanoparticles sufficiently bioactive for incorporation into pharmaceutical gels. Methods: Silver nanoparticles were prep...