Effect of garlic (Allium sativum) on lipid profiles, antioxidant activity and expression of scavenger receptor class B type I (SR-BI) in liver and intestine of hypercholesterolemic mice
Journal Title: JOURNAL OF ADVANCES IN CHEMISTRY - Year 2013, Vol 5, Issue 3
Abstract
Garlic is one of the medicinal plants, which has shown many useful effects, including Antioxidant activity, hypolipidemic and hypocholesterolemic effects, anti-hypertensive and anti-diabetic effects. Scavenger receptor class B type I (SR-BI) is a cell-surface receptor which mediated selective cholesterol ester uptake from the HDL particle. SR-BI also has an important role in cholesterol absorption in the intestine. Animals were randomly divided into three groups (n=8); group 1: received chow + cholesterol (2%) + cholic acid (0.5%), group 2: chow + 4% (w/w) garlic extract + cholesterol (2%) + cholic acid (0.5%), and group 3: chow only. After one-month mice were sacrificed, blood was collected; lipid profile and blood glucose were determined enzymatically as well as mRNA and protein levels of SR-BI were determined by RT-PCR and westernblot respectively. Compared with hypercholesterolemic control, garlic extract significantly decreased total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides, Very Low-Density Lipoprotein-Cholesterol (VLDL-C) and atherogenic index (all of them P<0.05). The activity of  Super oxide dismutase(SOD), glutathione (GSH)  and catalase levels were markedly increased in garlic-treated animals compared with hypercholesterolemic animals (p<0.05). The plasma levels of MDA markedly reduced in garlic group compared with hypercholesterolemic group. Intestinal SR-BI mRNA and protein were significantly decreased in garlic extract mice treatment compared with hypercholesterolemic control. Levels of liver SR-BI protein significantly reduced in hypercholesterolemic group (P<0.05). In conclusion, garlic extract markedly reduced TC, LDL-C, TG, VLDL-C and atherogenic index, as compared with the hypercholesterolemic control group. On the other hand garlic extract led to down-regulation of SR-BI protein and mRNA in the intestine of mice.
Authors and Affiliations
Abbas Mohammadi, Parham Norouzian, Mohammad Jamshidi, Nader Najafi, Ebrahim Abbasi Oshaghi
Keywords
Related Articles
- EP ID EP652574
- DOI 10.24297/jac.v5i3.2665
- Views 175
- Downloads 0
Intercalated nanosized MO2 (M: Sn, Ce) layers between CNTs and Pt or PtSn nanoparticles catalysts
Direct ethanol fuel cells (DEFCs) is receiving enormous attention as alternative electrical energy conversion systems. This paper gives an outline on some recent advances achieved in our laboratory regarding the developm...
THD ANALYSIS IN THREE PHASE-THREE LEVEL VSI WITH MPPT TRACKER AND SEPIC CONVERTER FOR SOLAR PV ARRAY
This paper deals with the analysis of total harmonic distortion in three phase three level voltage source inverter (VSI) for the solar photovoltaic array. The Voltage source inverter as used in high power applications he...
PHYTOCHEMICALS, VITAMINS,MACRO AND MICRO ELEMENTS AND ANTIMICROBIAL ANALYSIS OF THE STEM BARK OF NAPOLEONA VOGELII(AKPAESU)
Sequel to the increasing application of plant materials in ethnomedicine and for nutritive purposes the phytochemicals, vitamins, macro and micro elements and antimicrobial analysis of the stem bark of Napoleonavogelii w...
Accelerated Short-Term Techniques to Evaluate Corrosion in TiC Reinforced AA6063 Composites
AA6063-TiC composites have several weight percentages up to 9 wt. % were fabricated by using stir casting route method. The effects of the weight percentage of TiC particles on the microstructures and corrosion behavior...
An Improved Fire Fly Algorithm to Solve Economic Load Dispatch Problem including Practical Constraints
The main objective of Economic Load Dispatch Problem of power generation is to schedule the committed generating units optimally so as to meet the required load demand while satisfying all the units with equal and inequa...